ORIGINAL ARTICLE
Year : 2012  |  Volume : 11  |  Issue : 1  |  Page : 42-48

Production and properties of dextransucrase by free and immobilized cells of Leuconostoc paramesenteroides


1 Department of Natural and Microbial Products Chemistry, National Research Center, Dokki, Egypt
2 Faculty of Sciences, Department of Microbiology, Ain Shams University, Cairo, Egypt

Correspondence Address:
Amal M. Hashem
MD, Department of Natural and Microbial Products Chemistry, National Research Center, Tahrir Street, Dokki, 12311, Cairo
Egypt
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Source of Support: None, Conflict of Interest: None


DOI: 10.7123/01.EPJ.0000415613.02268.4d

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Six bacterial strains were tested for the production of dextransucrase. The highest dextransucrase activities were produced by local strain Leuconostoc paramesenteroides after 24 h in static culture conditions. Dextransucrase was induced using sucrose (10 g%) as a carbon source and corn steep liquor (3.5 ml%) as a nitrogen source. Fe2+ favoured enzyme production. Cells of L. paramesenteroides were immobilized using three supports, calcium alginate, agar and κ-carrageenan. Calcium alginate beads were able to produce dextransucrase for four runs with 100% activity. The optimum temperature for enzyme reaction ranged from 30 to 65πC at pH 5.4. In the absence of substrate, the enzyme retained 100% activity for 30 min at 40πC and 69% activity for 10 min at 60πC.



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