ORIGINAL ARTICLE
Year : 2013  |  Volume : 12  |  Issue : 1  |  Page : 40-45

Biotransformation of soybean saponin to soyasapogenol B by Aspergillus parasiticus


1 Department of Chemistry of Natural and Microbial Products, National Research Center, Dokki, Egypt
2 Department of Microbiology, Faculty of Science, Ain shams University, Cairo, Egypt

Correspondence Address:
Hala A. Amin
PhD, Department of Chemistry of Natural and Microbial Products, National Research Center, Dokki, 12311 Cairo
Egypt
Login to access the Email id

Source of Support: None, Conflict of Interest: None


DOI: 10.7123/01.EPJ.0000427332.31862.10

Rights and Permissions

Objectives

The aim of this study was to select of the most potent fungus that is able to hydrolyze soybean saponin (SS) to soyasapogenol B (SB). The selected fungus was cultivated under different physiological conditions to evaluate its ability to transform SS to achieve the maximal conversion output.

Materials and methods

Within 72 h, the biotransformation of SS by Aspergillus parasiticus, followed by isolation and purification of SB as a main product were carried out. The identity of SB was established by determination of its RF value and IR, mass spectra, and NMR spectra. Furthermore, different sets of experiments were carried out to enhance the activity of the tested organism and consequently, SB production.

Results and conclusion

Screening of different fungal isolates for transformation of SS to SB revealed that A. parasiticus produced the highest yield of SB. The maximum SB yield was obtained using a production medium composed of (%, w/v): malt extract, 4; yeast extract, 2; KH2PO4, 0.2; (NH4)2SO4, 0.2; MgSO4·7H2O, 0.03; CaCl2·2H2O, 0.03; galactose, 0.5; and SS, 3 (pH 8). The medium was inoculated with 6% (v/v) inoculum of a 72 h old culture and incubated on a rotary shaker (150 rpm) at 30πC for 72 h. Under these optimal conditions, the cell biotransformation efficiency was increased from 13.44 to 65%.



[FULL TEXT] [PDF]*
Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)
 

 Article Access Statistics
    Viewed1006    
    Printed39    
    Emailed0    
    PDF Downloaded145    
    Comments [Add]    

Recommend this journal