ORIGINAL ARTICLE
Year : 2013  |  Volume : 12  |  Issue : 2  |  Page : 136-141

Synthesis of the nonapeptide (B 22−B 30 ) of insulin B-chain using liquid and modified solid-phase methods


1 Peptide Chemistry Department, National Research Centre, Dokki, Cairo, Egypt
2 Chemistry Department, Faculty of Science, Zagazig University, Zagazig, Egypt

Correspondence Address:
Mohamed A Zewail
Peptide Chemistry Department, National Research Centre, 12311 Dokki, Giza
Egypt
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/1687-4315.124014

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Background and objective This work embraced a systematic search for potentiated methodologies for peptide synthesis through a di-dimensional approach for convenient synthesis of the nonapeptide (B 22 -B 30 ) of porcine insulin B-chain. Liquid-phase peptide synthesis (LPPS) and liquid-solid-phase peptide synthesis (modified SPPS) were used for the synthesis of this active part. Materials and methods A nonapeptide Arg-Gly-Phe-Phe-Tyr-Thr-Pro-Lys-Ala-OH corresponding to B 22 -B 30 of porcine insulin B-chain was synthesized using different methods: LPPS and modified SPPS. Time consumption, yield and purity of all products using the different methods were compared with each other. Results and conclusion The results indicated that modified SPPS is advantageous, as it consumes less solvent per coupling and deprotection reaction, thereby reducing operating costs and solvent waste. Reducing side reactions result in racemization, cyclization or premature peptide formation. Modified SPPS produces peptides with yields and purity better than LPPS. Also, it can reduce the time of coupling and deprotection reactions.


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