|Year : 2013 | Volume
| Issue : 2 | Page : 163-172
Diketopiperazine derivatives from Enterobacter cloacae isolated from the Red Sea alga Cystoseira myrica
Noha A Mohammed1, Hossam M Hassan2, Mostafa E Rateb2, Eman F Ahmed3, Usama W Hawas4, Somayah Sameer5, Rainer Ebel5, Mounir M El-Safty6, Mohammed S Abdel Hameed1, Ola H Hammouda1
1 Department of Botany, faculty of Science, Beni-Suief University, Beni-Suief, Cairo, Egypt
2 Department of Pharmacognosy, College of Pharmacy, Beni-Suief University, Beni-Suief, Cairo, Egypt
3 Department of Chemistry of Natural and Microbial Products, National Research Centre, 12311, Dokki, Cairo, Egypt
4 Marine Chemistry Department, Faculty of Marine Sciences, King Abdulaziz University, Jeddah, Saudi Arabia
5 Marine Biodiscovery Centre, University of Aberdeen, Scotland, United Kingdom
6 Central Laboratory for Evaluation of Veterinary Biologics (CLVB), Cairo, Egypt
|Date of Submission||03-Jun-2013|
|Date of Acceptance||26-Aug-2013|
|Date of Web Publication||31-Dec-2013|
Eman F Ahmed
Department of Chemistry of Natural and Microbial Products, National Research Centre, Dokki, 12311 Cairo
Source of Support: None, Conflict of Interest: None
This study is an attempt to explore the biological activities of isolated endophytic bacteria from marine sources that were coded A1, A2, and A3 (Padina pavonica), A4 (Cystoseira myrica), A5 (Acanthophora dendroides), and A6 (Sargassum sabrepandum). The bacteria coded C1, C2, and C3 were isolated from the soft coral Nephthea mollis and S1 and S2 were isolates from the sponge Hymedesmia spp. The primary aim of the study was the identification of active compounds.
Materials and methods
The bioactive compounds were extracted using ethyl acetate from nutrient broth media; biological activities of the extracted metabolites and 16S rDNA identification of the most promising isolate were studied. The eight major fractions of the extract showed different composition patterns when identified by liquid chromatography/mass spectrometry analyses.
Results and conclusion
Agar diffusion assay showed inhibitory activities of A4 extracts against the growth of most pathogenic microorganisms. Identification using PCR 16S rDNA and electrophoresis confirmed 98% identity to the Enterobacter cloacae strain GH1 (ac: JF261136.1). Eight compounds out of fifteen in the extract were identified as diketopiperazine derivatives. The maximum growth of E. cloacae was obtained at 30°C, pH 7, with the addition of maltose and KI to the media. The free radical scavenging activity exhibited good antioxidant activity (72.19%, IC 50 = 1.266 mg/ml) on using 2.0 mg/ml of the crude extract. The extract showed a high antiviral activity towards Newcastle disease virus and avian influenza virus A(H5N1).
Keywords: antimicrobial activity, antioxidant, antiviral, diketopiperazines, Enterobacter cloacae
|How to cite this article:|
Mohammed NA, Hassan HM, Rateb ME, Ahmed EF, Hawas UW, Sameer S, Ebel R, El-Safty MM, Abdel Hameed MS, Hammouda OH. Diketopiperazine derivatives from Enterobacter cloacae isolated from the Red Sea alga Cystoseira myrica. Egypt Pharmaceut J 2013;12:163-72
|How to cite this URL:|
Mohammed NA, Hassan HM, Rateb ME, Ahmed EF, Hawas UW, Sameer S, Ebel R, El-Safty MM, Abdel Hameed MS, Hammouda OH. Diketopiperazine derivatives from Enterobacter cloacae isolated from the Red Sea alga Cystoseira myrica. Egypt Pharmaceut J [serial online] 2013 [cited 2017 Dec 17];12:163-72. Available from: http://www.epj.eg.net/text.asp?2013/12/2/163/124030
| Introduction|| |
Marine organisms are an inexhaustible source of useful chemical substances for the development of new drugs; among these organisms are marine algae, which are capable of biosynthesizing a broad range of secondary metabolites, and bacteria that live in oceans. Enterobacter cloacae is a rod-shaped, Gram-negative bacterium that frequently grows at 30°C on nutrient agar medium (NAM) or nutrient broth medium  . It is a member of the normal gut flora in many humans  and has been used as a biological control in plant diseases such as those caused by the seed-rotting oomycete Pythium ultimum. It is also used to control insect pests on mulberry leaves and suppress disease  .
Many of the marine algae species are often accompanied by several bacterial strains that are obtained from the sea together with the algae cells, or are the result of contamination of the algae culture. These mixed populations that are present in the culture and in the sea show that the bacteria use organic substances secreted by living or dead algae cells. It has been observed that many types of seaweed thrive in the presence of bacteria than in their absence. Some seaweed species need vitamins for their growth and possibly the bacteria are partially responsible for the production of these substances; some of them even produce antibiotics , . Because of the highly chemical and harsh physical conditions in the marine environment, the organisms produce a variety of molecules with unique structural features and exhibit various biological activities. The majority of the marine natural products have been isolated from sponges, coelenterates (sea whips, sea fans, and soft corals)  .
As antibiotic resistance has developed, medical researchers have fought back with alternative antibiotics and combination therapy, but constant overuse of antibiotics in humans and livestock have lead to bacterial strains becoming resistant to nearly all known antibiotics. Hence, constant effort is required to discover newer antibacterial agents that are effective against pathogenic bacteria that are resistant to currently available antibiotics  .
| Materials and methods|| |
Collection and identification of samples
Sponge, soft coral, and seaweeds were collected from the Red Sea at a depth of 5-8 m from the coast of Rass Mohammed (South Sinai, Egypt) and at a depth of 2 m from the Institute of Marine Science (Hurghada, Egypt) in March 2010. The samples were washed with tap water and identified by the Coral Reef Ecology and Biology Group, National Institute of Oceanography and Fisheries (Suez, Egypt).
Isolation and maintenance of endophytic isolates
The marine organism was soaked in distilled water, drained, and then rinsed in 70% ethanol for 30 s, followed by wash in sterile water; tissues were cut into 0.5-1 cm pieces. The sterile pieces were placed on the surface of NAM containing the following components (g/l): glucose 10, peptone 6, yeast extract 3, meat extract 1.5, and agar 25. The media were incubated for 28 h at 30°C. On the basis of morphological features, colonies were picked, purified, and maintained on the NAM.
Extraction of bioactive compounds
The isolated bacteria were inoculated in 20 l nutrient broth medium at 30°C under shaking conditions and centrifuged after 4 days. The filtrate and the separated cell mass were extracted three times by ethyl acetate and evaporated until they were completely dry using a rotavapor with heated waterbath up to 40°C. The crude extract was weighed and tested for biological activities.
All the tested organisms were supplied by the Biotechnological Research Center, Al-Azhar University (Cairo, Egypt); the bacteria were maintained on NAM and the yeast on potato dextrose agar (PDA) medium (peeled potato 200 g, dextrose 20 g, agar 18 g in 1000 ml distilled water).
Gram-positive bacteria comprised Bacillus subtilis (NCTC 1040), Staphylococcus aureus (NCTC 7447), and Sarcina maxima (ATCC 33910); Gram-negative bacteria comprised Escherichia More Details coli (NCTC 10416) and Pseudomonas aeruginosa (ATCC 10145); and the yeast was Candida albicans (IMRU 3669).
| In-vitro antagonism assay|| |
A definite concentration of the crude extract (1 mg/ml) was prepared, 10 μl was used to saturate a 6 mm filter paper (Whatman 4) and was placed in Petri dish More Detailses coated with NAM for bacteria and PDA for yeast. Both media were previously inoculated with a 0.1 ml spore suspension of the pathogens  .
The previously mentioned bacteria were incubated overnight at 37°C for 24 h, whereas yeast was cultured at 28°C for 4 days. The inhibition zones were measured in mm.
To assess the role of the salinity and components in the media on the production of bioactive metabolites, the promising isolated bacterium was cultivated in 250 ml flasks containing 100 ml of the following media:
Media I (50% seawater), containing (g/l): yeast extract 3, glucose 10, meat extract 1.5, and peptone 6.
Media II (50% seawater), containing (g/l): yeast extract 4, glucose 4, and malt extract 10.
Media III (100% seawater), containing (g/l): yeast extract 4, glucose 4, and malt extract 10.
Media IV (50% seawater), containing (g/l): peptone 5 and yeast extract 1.
Media V (100% seawater) containing (g/l): peptone 5 and yeast extract 1.
PCR 16S rDNA and electrophoresis
DNA extraction and PCR amplification of the 16S rDNA region were carried out. DNA was isolated from the isolates (A4) according to the method prescribed by Sambrook et al.  . The 16S rDNA was amplified by PCR using primers designed to amplify the 1500 bp fragment of the 16S rDNA region. The forward primer was 5Ͳ-AGAGTTTGATCMTGGCTCAG-3Ͳ and the reverse primer was 5Ͳ-TACGGYTACCTTGTTACGACTT-3Ͳ. The PCR mixture consisted of 30 pmol of each primer, 10 ng of chromosomal DNA, 200 μmol/l dNTPs, and 2.5 U of Taq polymerase in 50 μl of polymerase buffer. The PCR was carried out for 30 cycles at 94°C for 1 min, at 55°C for 1 min, and at 72°C for 2 min. After completion, a fraction of the PCR mixture was examined using agarose gel electrophoresis  . DNA sequences were obtained using a 3130X DNA Sequencer (Genetic Analyzer; Applied Biosystems, Hitachi, Japan) and BigDye Terminator Cycle Sequencing (see details below). The PCR product was sequenced using the same PCR primers. Blast program was used to assess the DNA similarities and multiple sequence alignment and molecular phylogeny were performed using BioEdit software  .
Automated DNA sequencing based on the enzymatic chain terminator technique, developed by Sanger et al.  , was performed using the 3130X DNA Sequencer (Genetic Analyzer; Applied Biosystems). The sequencing reaction was performed with four different fluorescent labels identifying the ddNTPs instead of the radioactive labels. These fluorophores were excited with two argon lasers at 488 and 514 nm when the respective bands passed the lasers during the electrophoresis. The specific emissions were detected and the data were collected for analysis , . The thermal cycling mixture was as follows: 8 μl of BigDye Applied Biosystems, Foster City, California 94404, USA terminator mix, 6 μl of the sequencing primer (10 pmol), and 6 μl of the sample (PCR product or plasmid); the reaction was then run in the thermal cycler. The cyclic reaction consisted of 1 min at 95°C, followed by 49 cycles of 30 s at 95°C, 10 s at 52°C, and 4 min at 60°C. The products were purified using a special column according to the instructions of the manufacturer. The elute was taken and high dye formamide was added at 1 : 1/volume ratio; the reaction was run at 95°C for 5 min for denaturation, shock on ice, after which the sample was ready for sequencing and analysis in the 3130X DNA sequencer.
Antioxidant activity (DPPH assay)
The free radical scavenging activity using 1,1-diphenyl-2-picryl-hydrazil (DPPH) reagent was determined according to Brand-Williams et al.  . The bacterial extract was dissolved in methanol to prepare different concentrations. To each 1.5 ml of the extract sample 1.5 ml of freshly prepared methanolic DPPH solution (20 μg/ml) was added and stirred. The decolorizing process was recorded after 5 min of reaction at 517 nm and compared with a blank control.
Antioxidant activity = control absorbance - sample absorbance/control absorbance × 100%
The virus and media
Highly pathogenic avian influenza HPAI H5N1 virus A (Chicken/Egypt/9402 NAMRU 3-CLEVB 213/2007) (H5N1) of accession number Eu 623467 obtained from control Laboratory for Evaluation of Veterinary Biologics (CLEVB) was used at a titer of 106 EID 50 /ml.
Growth medium (minimum essential medium; Gibco China (Mainland)) was supplemented with 10% new-born calf serum at pH 7.2 for primary and secondary cultures. Trypsin (1-250; Gibco) was used at a concentration of 0.23% as a cell dispersing agent. Cells of African green monkey kidney cells (Vero cells)  and chicken embryonic fibroblasts were cultured.
Specific pathogen-free (SPF) 9-day-old embryonated chicken eggs (ECEs) were inoculated via the allantoic sac route.
Toxicity using embryonated chicken eggs
ECEs of 9 days old (130 SPF) and six-fold dilutions of the total extract were used, 0.2 ml/ECE of each dilution was inoculated into five ECEs through allantoic sac allantoic sac and incubated at 37°C with 80% humidity for 5 days; through all eggs candled every day.
Various concentrations of the extract were added to confluent chicken embryonic fibroblast cells and Vero cell monolayer cultures 24 h after seeding and the cultures were maintained for 4 days. Then viable cell counts of each culture were determined daily by inverted microscopic inspection of cells not affected by compounds.
Hemagglutinating activity assay
Hemagglutinating activity assay was performed on the allantoic fluids of the inoculated eggs and the activity was measured by means of a microtechnique  .
SPF ECEs were inoculated with equal volumes of Newcastle disease virus (NDV) and a nontoxic concentration after incubation of the mixture for 1 h at room temperature. The mixture was inoculated into allantoic sacs of five ECEs at 0.2 ml/ECE. Five ECEs were inoculated with the NDV, which was mixed with an equal volume of saline at 0.2 ml/ECE (positive control); another five ECEs were inoculated with saline alone at 0.2 ml/ECE (negative control). Thereafter, all the ECEs were inoculated at 37°C and candled every 2 h until all positive control ECEs had died.
Using a medium pressure liquid chromatography column, with normal silica gel 60, gradient elution was carried out starting with 100% n-hexane and ending with 100% ethyl acetate. Thin layer chromatography monitoring of different fractions was carried out using hexane : ethyl acetate (S1 8 : 2, S2 7 : 3, S3 5 : 5), and spots were visualized using ultraviolet and P-anisaldehyde/H 2 SO 4 spray reagent.
Liquid chromatography/mass spectrometry analyses
High-resolution mass spectrometric (MS) data were obtained using a Thermo Instruments MS system (LTQ XL/LTQ Orbitrap Discovery: Thermo Scientific, Oxford, UK. coupled to a Thermo Instruments HPLC system (Accela PDA detector, Accela PDA autosampler, and Accela pump) TUV Rheinland of North America Inc.) under the following conditions: capillary voltage 45 V, capillary temperature 260°C, auxiliary gas flow rate 10-20 AU, sheath gas flow rate 40-50 AU, spray voltage 4.5 kV, and mass range 100-2000 a.m.u. (maximum resolution 30 000). Structure elucidation of the isolated compounds was carried out on the basis of comparison of their NMR spectral data as well as the HRESI-MS of these compounds with the literature data and differert compound libraries.
Physiological studies on the isolated endophyte
Effect of temperature on the growth of bacteria
0The bacterium was incubated statically in NAM at different temperatures (40, 50, 60, and 70°C) for 28 h to determine the optimum temperature for growth.
The role of pH levels on the growth
Nutrient broth media (50 ml in 250 ml flasks) were adjusted to cover the pH range (3, 4, 5, 6, 7, 8, 9, and 10) using 0.1 mol/l NaOH and 0.1 mol/l HCl and buffer solutions. Spore suspension (1 ml) was inoculated in the flasks and shaken for 24 h. Absorbance was read at 600 nm  .
Influence of different salts
Peptone 0.3, yeast extract 0.15, and glucose 0.5 (g/l) were added to 50 ml of 0.1 mol/l) each of the following salt solutions: FeCl 3 , Fe 2 SO 4 , CuSO 4 , K.phthalate, ZnSO 4 , EDTA.2Na, Na 2 HPO 4 , KH 2 PO 4 , MgSO 4 0.7H 2 O, NaCl, CaCl 2 , KCl, KI, CH 3 COONa.3H 2 O, Na 2 HPO 4 , NaHCO 3 , Na 2 CO 3 , and MnCl 2 . Spore suspension (1 ml) was used to inoculate the medium, which was shaken at 30°C, and the absorbance was read at 600 nm after 28 h.
Significance of different carbon sources on growth production
Carbon sources such as galactose, glucose, raffinose, lactose, mannose, cellobiose, manitol, glycerol, sucrose, and maltose were used in weights equivalent to the carbon amounts in 2 g of glucose and added separately to 50 ml of medium that contained the following (g/l): yeast extract 0.5, KH 2 PO 4 0.1, and MgSO 4 .7H 2 O 0.5. The media were inoculated with 1 ml of spore suspension, shaken for 28 h, and absorbance read at 600 nm.
| Results and discussion|| |
Isolation and antimicrobial potential of the marine bacteria
On the basis of the distinct colony morphology of shape, size, and color, 11 endophytic bacteria were isolated from seaweeds, soft corals, and sponge [Table 1]. The antagonistic potential of the metabolites in the total extract (300 μg/disc) was tested against six pathogenic organisms [Table 2]. Among the 11 isolated endophytes, it is evident that A4 was the most active against the most tested microorganism. Depending on this outcome, A4 was chosen for further biological assessments.
|Table 2: The antimicrobial screening of the isolated extract (300 μ g/disc) |
Click here to view
Isolation, identification, and phylogenetic analysis
Only one isolate, A4, was selected as a promising producer of bioactive compounds. Molecular identification of this isolate based on 16S rDNA sequencing was carried out. A fraction of the PCR mixture was examined using agarose gel electrophoresis [Figure 1]. According to sequencing similarities and multiple alignment, the isolate A4 was found to be closely related to E. cloacae strain GH1 (ac: JF261136.1) with a 98% identity. The phylogenetic tree was displayed using the TreeView program [Figure 2]  .
Influence of culture composition on the production of bioactive metabolites
To detect the role of cultivation media and salinity on the production of active metabolites, five media were examined. It was clearly obvious that media I, which was mainly composed of 10 g glucose in 50% seawater, was the most favorable for the production of bioactive compounds and showed inhibition against most pathogens [Figure 3], [Figure 4], [Figure 5], [Figure 6] and [Figure 7]. In contrast, the decrease in glucose concentration in media II and III led to weak activity [Figure 4] and [Figure 7] or a complete loss of activity [Figure 3] and [Figure 5]. The presented data proved that S. maxima was the most sensitive strain to the metabolites produced in the fermentation medium (I) by E. cloacae at all concentrations used [Figure 4], followed by S. aureus [Figure 5]. The data also showed sensitivity of B. subtilis to the metabolites in media IV [Figure 3]. These results indicated the significance of carbon source concentrations and salinity in the production of bioactive compounds. To the best of our knowledge the biological activity of E. cloacae has not been investigated before. Seventy-five marine bacterial strains from sponges were isolated to evaluate the antimicrobial potential; 21% of the bacterial strains were found to be antibiotics producers and their activities ranged from broad spectral to species specific  . In addition, 12 isolates from Brazilian sponges presented antimicrobial activities against Bacillus spp., Virgibacillus spp., Pseudovibrio spp., Pseudomonas spp., and Stenotrophomonas spp.  . An antibiotic producer Pseudomonas spp., from the Persian Gulf, was also investigated  .
The results in [Table 3] showed good antioxidant activity for the endophyte extract at a concentration of 2 mg/ml (IC 50 was obtained at 1.266 mg/ml). In contrast, the other concentrations revealed a weak activity. To our knowledge this is the first report on the antioxidant activity of E. cloacae metabolites. The antioxidant activity of the metabolites produced by the marine bacteria Virgibacillus spp. associated with the sponge Callyspongia diffusa was studied  . This research revealed the potential of marine sponge-associated bacterial bioactive compounds in scavenging free radicals in vitro.
|Table 3: Antioxidant activity of Enterobacter cloacae extracts using DPPH assay|
Click here to view
Our results on the antioxidants from endophytic bacteria were supported by those of Takao et al.  , who isolated 112 marine bacterial strains with antioxidant activity. Further, Krishna et al.  focused on the antioxidant potential of marine B. subtilis (MTCC no. 10619). They concluded that the production of catalase enzyme, nonenzymatic parameters, and ascorbic acid was responsible for the activity.
This extract was evaluated for in-vitro antiviral activity. The present findings showed that all the embryos of the positive controls (inoculation of the virus without the extract) had died and the allantoic fluid of each was positive in the hemagglutination assay, whereas the inoculation of the bacterium extract with H5N1 and NDV viruses inhibited the virus effects [Table 4]. A marked decrease in hemagglutination and in the number of dead embryos was observed. The results obviously showed that the bacterium was effective against both respiratory RNA virus (H5N1) and NDV. The resistance of viral pathogens to existing treatment drugs has increased the demand for new medicines. The screening of natural products derived from marine species for antiviral activity has yielded a considerable number of active crude aqueous and organic solvent extracts. Today, over 40 compounds are commercially available in pharmacological markets, including alternative antiviral medicines  . A team of research scientists evaluated the antiviral and immunoregulatory effect of a novel exopolysaccharide (EPS-1) from a marine thermotolerant Bacillus licheniformis and found that EPS-1 treatment impaired HSV-2 replication in human peripheral blood mononuclear cells  . In contrast, marine bacteria with antiviral properties have been isolated and tentatively classified as Moraxella More Details. They concluded that the antiviral capacity of the isolated microorganisms was highly specific for poliovirus  .
Fractionation and identification of the active compounds
The literature review revealed no information on the chemistry of the Enterobacter spp. extract. The medium pressure liquid chromatography column, with silica gel 60, yielded 120 fractions. Thin layer chromatography monitoring of different fractions was carried out using hexane : ethyl acetate (S1 8 : 2, S2 7 : 3, S3 5 : 5), and spots were visualized using ultraviolet and P-anisaldehyde/H 2 SO 4 spray reagent. Eight major fractions were collected (F1-F8) [Table 5]. LC/HRESI-MS of the collected fractions was carried out showing the chemical pattern of each fraction. Eight compounds out of 15 were identified by comparing the MS/MS fragmentation pattern with the library database. Data showed that around 18 compounds were repeated in different fractions.
|Table 5: Liquid chromatography/mass spectrometry data of Enterobacter fractions with the suspected formula and suggested identified compounds|
Click here to view
The identified compounds were found to be localized in fractions F6, F7, and F8. It was clear in [Table 6] that the following identified compounds in fractions 6 and 7 had weak activities against the examined microorganisms: cyclo (leucylprolyl), cyclo (l-phe-l-pro), cyclo (prolylvalyl), cyclo (l-phe-l-pro), and l-leucine anhydride. In contrast, a moderate activity was seen for cyclo (tyrosyl-prolyl), cyclo (d-hypro-l-leu), and d,d-4-hydroxyprolyl-phenylalanyl in F8; these compounds were responsible for the antipathogenic activity against most microbes. The unidentified compounds revealed a range of molecular formulae, but the presence of nitrogen was a common feature among most of them, which could lead to the discovery of new diketopiperazine or other peptide derivatives as published before  .
|Table 6: Antimicrobial activity of eight fractions (150 mg/disc) of Enterobacter spp. against some pathogenic microorganisms|
Click here to view
In this study we tried to ascertain the appropriate conditions for E. cloacae growth. It was obvious that the cells were resistant to very low and high temperatures (20 and 50°C) and it was noticed that the favorable conditions for maximum growth of the isolated bacterium was 30°C [Table 7] and pH 7 [Figure 8]. The alkaline media at pH 8 and 9 also aided in good germination of the microorganism. Reviewing the literature, a study on the interactive effect of temperature and pH on EPS production by Enterobacter spp. A47 was reported. The results revealed the ability of Enterobacter spp. A47 to synthesize different heteropolysaccharides as a function of pH and temperature, and this synthesis was stable for wide pH and temperature ranges  . In contrast, the optimum temperature for the growth of the facultative anaerobe Enterobacter aerogenes was about 40°C at pH 7.0  .
|Table 7: Effect of different temperatures on the growth of Enterobacter cloacae |
Click here to view
Endophyte growth was evaluated in media supplemented with different carbon sources and salts. The results in [Table 8] show that maltose was the most favorable nutrient for the organism, followed by mannose (96.8%) and raffinose (89.6%). The addition of potassium salts (KI and KCl) to cultivation media gave the most favorable results, followed by CaCl2 [Table 9]. The growth responses of the marine organisms when various groups of salts were omitted in turn from artificial seawater were evaluated. It was evident that removing either Na + or K + salts completely hampered growth in all cases  . It was reported that the optimal temperature for the growth of Enterobacter spp. is between 30 and 37°C and the microbe has a fermentative type of metabolism. d-Glucose and other carbohydrates are catabolized with the production of acid and gas. The carbohydrates that are fermented by Enterobacter spp. include mostly cellobiose, l-arabinose, d-mannitol, maltose, d-mannose, trehalose, and salicin  .
|Table 8: Effect of different carbon sources on the growth of Enterobacter cloacae |
Click here to view
|Table 9: The role of different salts on the growth of Enterobacter cloacae |
Click here to view
| Conclusion|| |
This article focused on the bioactive potential of the metabolites produced by E. cloacae, which revealed a promising inhibition against pathogenic bacteria and H5N1 and NDV viruses. Antioxidant activity was detected in the total extract of the marine bacterium from which diketopiperazines derivatives were isolated. This study explores the possibility for the discovery and use of new drugs from natural sources.
| Acknowledgements|| |
The authors thank Assistant Professor Lamia Abu El-Kassem for her kind assistance in the practical work.
Conflicts of interest
There are no conflicts of interest.
| References|| |
|1.||Dalben M, Varkulja G, Basso M, Krebs VL, Gibelli MA, van der Heijden, I, et al. Investigation of an outbreak of Enterobacter cloacae in a neonatal unit and review of the literature. J Hosp Infect 2008; 70:7-14. |
|2.||Keller R, Pedroso MZ, Ritchmann R, Silva RM. Occurrence of virulence-associated properties in Enterobacter cloacae. Infect Immun 1998; 66:645-649. |
|3.||Van Dijk, K Nelson, EB Fatty acid competition as a mechanism by which Enterobacter cloacae suppresses Pythium ultimum sporangium germination and damping-off. Appl Environ Microbiol 2000; 66:5340-5347. |
|4.||Jasti S, Sieracki ME, Poulton NJ, Giewat MW, Rooney-Varga, JN Phylogenetic diversity and specificity of bacteria closely associated with Alexandrium spp. and other phytoplankton. Appl Environ Microbiol 2005; 71:3483-3494. |
|5.||Penesyan A, Kjelleberg S, Egan S. Development of novel drugs from marine surface associated microorganisms. Mar Drugs 2010; 8:438-459. |
|6.||Mercado IES, Gómez JV, Rivas GG, Sánchez NEA2012. Bioactive compounds from bacteria associated to marine algae (chapter 3). Biotechnology - Molecular Studies and Novel Applications for Improved Quality of Human Life. Sammour RH, editor. 2012. p. 25-44. |
|7.||Ravikumar S, Thajuddin N, Suganthi P, Jacob Lnbaneson, S Vinodkumar, T. Bioactive potential of seagrass bacteria against human bacterial pathogens. J Environ Biol 2010; 31:387-389. |
|8.||Cosentino S, Tuberoso CIG, Pisano B, Satta M. Spoilage organisms. Int J Food Microbiol 1999; 155-162. |
|9.||Sambrook J, Fritsch EF, Maniatis T. Molecular cloning: a laboratory manual. 1989; NY: Cold Spring Harbor Laboratory. |
|10.||Ausubel FM, Brent R, Kingston RE, More DD, Seidam JG, Smith JA, Struhl K (editors) Short protocols in molecular biology. 1999;NY: John Willey and Sons Inc.; 29:130-135. |
|11.||Hall TA BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucl Acid Symp 1999; 41:95-98. |
|12.||Sanger F, Nicklen S, Coulson AR. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 1977; 74:5463-5467. |
|13.||Prober JM, Trainor GL, Dam RJ, Hobbs FW, Robertson CW, Zagursky RJ, et al. A system for rapid DNA sequencing with fluorescent chain-terminating dideoxynucleotides. Science 1987; 238:336-341. |
|14.||Freeman M, Baehler C, Spotts S. Automated laser-fluorescence sequencing. Bio/Technology 1990; 8:147-148. |
|15.||Brand-Williams W, Cuvelier ME, Berset C. Use of a free radical method to evaluate antioxidant activity. LWT Food Sci Technol 1995; 28:25-30. |
|16.||Yasumura Y, Kawakita M. The research for the SV40 by means of tissue culture technique. Nippon Rinsho 1963; 21:1201-1219. |
|17.||Takatsy G. The use of spiral loops in serological and virological micro-methods. Acta Microbiol Acad Sci Hung 1955; 3:191-202. |
|18.||Vasudevan DM, Vasudevan DM, Sreekumari S, Vaidyanathan K. Textbook of biochemistry for medical students 6th ed. 2010; New Delhi: Jaypee Bros. Medical Publishers. |
|19.||Page, RD TreeView: an application to display phylogenetic trees on personal computers. Comput Appl Biosci 1996; 12:357-358. |
|20.||Anand TP, Bhat AW, Shouche YS, Roy U, Siddharth J, Sarma SP. Antimicrobial activity of marine bacteria associated with sponges from the waters off the coast of South East India. Microbiol Res 2006; 161:252-262. |
|21.||Santos OCS, Pontes PVML, Santos JFM, Muricy G, Giambiagi-deMarval M, Laport MS. Isolation, characterization and phylogeny of sponge-associated bacteria with antimicrobial activities from Brazil. Res Microbiol 2010; 161:604-612. |
|22.||Darabpour E, Ardakani MR, Motamedi H, Ghezelbash G, Ronagh MT, Isolation of an antibiotic producer Pseudomonas sp. from the Persian Gulf. Asian Pac J Trop Med 2010; 3:318-321. |
|23.||Arunachalam K, Appadorai RAJ. Antioxidant potential and biochemical evaluation of metabolites from the marine bacteria Virgibacillus sp. associated with the sponge Callyspongia diffusa. Free Radic Antioxid 2013; 3:47-51. |
|24.||Takao T, Kitatani F, Watanabe N, Yagi A, Sakata K. A simple screening method for antioxidants and isolation of several antioxidants produced by marine bacteria from fish and shellfish. Biosci Biotechnol Biochem 1994; 58:1780-1783. |
|25.||Krishna ER, Kumar PS, Kumar BV. Study on antioxidant activity and strain development of Bacillus subtilis (MTCC No. 10619). J Agric Technol. 2011; 7:1693-1703. |
|26.||Yasuhara-Bell J, Lu Y. Marine compounds and their antiviral activities. Antiviral Res 2010; 86:231-240. |
|27.||Arena A, Maugeri TL, Pavone B, Iannello D, Gugliandolo C, Bisignano G. Antiviral and immunoregulatory effect of a novel exopolysaccharide from a marine thermotolerant Bacillus licheniformis. Int Immunopharmacol 2006; 6:8-13. |
|28.||Girones R, Jofre JT, Bosch A. Isolation of marine bacteria with antiviral properties. Can J Microbiol 1989; 35:1015-1021. |
|29.||Gao Y, Yu L, Peng C, Li Z, Guo Y. Diketopiperazines from two strains of South China Sea sponge-associated microorganisms. Biochem Syst Ecol 2010; 38:931-934. |
|30.||Torres CAV, Antunes S, Ricardo AR, Grandfils C, Alves VD, Freitas F, Reis MAM. Study of the interactive effect of temperature and pH on exopolysaccharide production by Enterobacter A47 using multivariate statistical analysis. Bioresour Technol 2012; 119:148-156. |
|31.||Tanisho S. Hydrogen production by facultative anaerobe Enterobacter aerogenes. BioHydrogen 1999; 273-279. |
|32.||MacLeod RA, Onofrey E. Nutrition and metabolism of marine bacteria II. Observations on the relation of sea water to the growth of marine bacteria. J Bacteriol 1956; 71:661-667. |
|33.||Pelczar MJ. Microbiology: concepts and applications. 1993; New York: McGraw-Hill Inc. |
[Figure 1], [Figure 2], [Figure 3], [Figure 4], [Figure 5], [Figure 6], [Figure 7], [Figure 8]
[Table 1], [Table 2], [Table 3], [Table 4], [Table 5], [Table 6], [Table 7], [Table 8], [Table 9]