ORIGINAL ARTICLE
Year : 2014  |  Volume : 13  |  Issue : 1  |  Page : 21-26

Synthesis of nonapeptide (B 22 -B 30 ) of insulin B-chain using modified solid-phase methods with and without microwave energy


1 Peptide Chemistry Department, National Research Centre, Dokki, Giza, Egypt
2 Chemistry Department, Faculty of Science, Zagazig University, Zagazig, Egypt

Correspondence Address:
Mohamed Aly Zewail
Ph.D, Peptide Chemistry Department, National Research Centre, Dokki 12311, Giza
Egypt
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/1687-4315.135594

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Background and objectives This work embraced a systematic search for potentiated methodology for peptide synthesis through an approach for convenient synthesis of the nonapeptide B 22 -B 30 of human insulin B chain. The modified solid-phase method with and without microwave technique was used for synthesis of this active part. Materials and methods Nonapeptide Arg-Gly-Phe-Phe-Tyr-Thr-Pro-Lys-Thr-OH corresponding to B 22 -B 30 of human insulin B chain was synthesized using modified solid-phase peptide synthesis. Time consumption, yields, and purity of all products using the different methods were compared with each other. Results and conclusion We can interpret that these results provide a bird's eye view of the benefits of microwave energy with solid phase peptide synthesis (SPPS) in the enhancement of coupling and deprotection reactions in peptide synthesis. In addition, the application of microwave energy with SPPS provides an efficient tool for peptide synthesis, as microwave energy can effectively disrupt intermolecular aggregation and prevent β-sheet formation. Microwave technique proved to be the better approach used for this purpose in the present work. In addition, the advantages of microwave energy include reduced side reactions that result in racemization, cyclization, or premature peptide formation. Microwave energy produces peptides with yields and purity better than the conventional method. It can accelerate the rate of coupling and deprotection reactions, reducing the reaction time from hours to minutes.


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