ORIGINAL ARTICLE
Year : 2015  |  Volume : 14  |  Issue : 3  |  Page : 158-165

Characterization of heavy metal and antibiotic-resistant bacteria isolated from polluted localities in Egypt


1 Department of Botany and Microbiology, Faculty of Science (Boys), Al-Azhar University, Cairo, Egypt
2 Department of Botany and Microbiology, Faculty of Science (Boys), Al-Azhar University, Cairo, Egypt; Department of Biology, Faculty of Science and Arts, Northern Borders University, Rafha, Kingdom of Saudi Arabia
3 Department of Natural and Microbial Products Chemistry, Division of Pharmaceutical and Drug Industries Research, National Research Center, Cairo, Egypt

Correspondence Address:
Saad A Moghannem
PhD, Department of Botany and Microbiology, Faculty of Science (Boys), Al-Azhar University, 11651 Cairo, Egypt

Login to access the Email id

Source of Support: None, Conflict of Interest: None


DOI: 10.4103/1687-4315.172856

Rights and Permissions

Objective The aim of this study was to isolate and identify heavy metal-resistant and antibiotics-resistant bacteria from contaminated samples (wastewater and soil) collected from different industrial areas in Egypt and determine their role in heavy metal removing. Materials and methods Samples were collected from Helwan and 10th of Ramadan city areas and enriched in culture broth containing 200, 100, and 10 ppm of arsenic (As), lead (Pb), and cadmium (Cd) as AsHNa 2 O 4·H 2 O, Pb(NO 3 ) 2 , and CdSO 4 , respectively. The highly resistant isolate (ST6) was selected and identified biochemically and also subjected to 16S rDNA sequencing. The growth parameters were optimized and the maximum tolerable concentration of the respective metals as well as the antibiotic resistance was determined. Result and conclusion After enrichment culture we isolated and purified 20 bacterial isolates resistant to the respective heavy metals As, Pb, and Cd. The morphological, biochemical, and phylogenetical characteristics of the most resistant bacterial isolates (ST6) were studied. The results showed that this isolate belongs to the species Pseudomonas stutzeri. The optimum temperature was 35°C, whereas the optimum pH was in the range of 6-7. Maximum tolerable concentration values for As, Pb, and Cd were 3500, 1750, and 50 ppm, respectively. Also, the isolate ST6 showed resistance against different antibiotics. The metal removal ability was 42.5, 57.1, and 52.9% of As, Pb, and Cd, respectively. It was concluded that the ST6 isolate was resistant and removed high concentrations of As, Pb, and Cd. Hence, this isolate may play a role in bioremediation processes of heavy metal in polluted areas.


[FULL TEXT] [PDF]*
Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)
 

 Article Access Statistics
    Viewed1398    
    Printed36    
    Emailed0    
    PDF Downloaded320    
    Comments [Add]    

Recommend this journal