|Year : 2015 | Volume
| Issue : 3 | Page : 180-186
Proximate analysis, phytochemical screening, and bioactivities evaluation of Cissus rotundifolia (Forssk.) Vahl. (Fam. Vitaceae) and Sansevieria cylindrica Bojer ex Hook. (Fam. Dracaenaceae) growing in Egypt
Ataa A Said1, Elsayed Ali Aboutabl2, Sally A El Awdan3, Mona A Raslan1
1 Department of Pharmacognosy, National Research Centre, Dokki, Giza, Egypt
2 Pharmacognosy Department, Faculty of Pharmacy, Cairo University, Cairo, Egypt
3 Department of Pharmacology, National Research Centre, Dokki, Giza, Egypt
|Date of Submission||24-Jan-2015|
|Date of Acceptance||17-Feb-2015|
|Date of Web Publication||30-Dec-2015|
Mona A Raslan
PhD, Pharmacognosy Department, National Research Centre, Dokki, 12622 Giza
Source of Support: None, Conflict of Interest: None
This work aimed to carry out a proximate analysis, phytochemical screening, and bioactivities evaluation of the nonflowering aerial parts of each of Cissus rotundifolia (Forssk.) Vahl. (Fam. Vitaceae) and S. cylindrica Bojer ex Hook. (Fam. Dracaenaceae) growing in Egypt.
Materials and methods
The total phenolic contents (TPC) and total flavonoid contents were estimated spectrophotometrically. The oral acute toxicity of each of the two plant extracts was evaluated. Analgesic activities of both plants were evaluated using hot plate and writhing test using indomethacin as a reference drug. Anti-inflammatory activities were evaluated using carrageenan-induced rat-paw edema. Antiulcerative activities were measured using indomethacin-induced ulcer in pyloric-ligated rats. Thioacetamide-induced acute hepatic encephalopathy was used to evaluate the hepatoprotective effects of the plants.
Results and conclusion
Proximate analysis of air-dried nonflowering aerial parts of each of C. rotundifolia and S. cylindrica yielded a moisture content of 7.95 and 7.52%, total ash of 8.35 and 9.83%, water-soluble ash of 3.96 and 2.94%, acid-insoluble ash of 2.14 and 3.42%, crude fiber content of 8.52 and 13.43%, and methanolic extractive value of 41 and 35%, respectively. Phytochemical analysis of C. rotundifolia extract indicated the presence of triterpenes, carbohydrates, and/or glycosides, tannins, flavonoids, coumarins, and saponins, whereas S. cylindrica extract showed the presence of sterols, carbohydrates, and/or glycosides, flavonoids, coumarins, and saponins. Total phenolic content of C. rotundifolia and S. cylindrica were 3.18 and 4.82 mg (GAE)/g, respectively, whereas total flavonoid contents of C. rotundifolia and S. cylindrica were 1.35 and 4.13 mg (QE)/g plant dry weight, respectively. Both extracts showed no toxicity up to 1 g/kg body weight. Both methanolic extracts showed significant central and peripheral analgesic effects. C. rotundifolia extract showed percentages of edema inhibition higher than or equal to the reference drug indomethacin in carrageenan-induced paw edema assay, whereas S. cylindrica extract showed percentages of edema inhibition less than that of the reference drug indomethacin. In the ulcer model induced by indomethacin, the groups pretreated with both plants received significant protection against ulcer formation. In the thioacetamide-induced acute hepatopathic encephalopathy model, rats treated with S. extract showed significant improvement in their plasma AST and ALT, whereas rats treated with C. rotundifolia extract showed increased levels of their ALT and AST.
Keywords: Analgesic, anti-inflammatory, antiulcerative, Cissus rotundifolia, flavonoids, hepatoprotective, phenolics, preliminary phytochemical screening, proximate analysis, Sansevieria cylindrica
|How to cite this article:|
Said AA, Aboutabl EA, El Awdan SA, Raslan MA. Proximate analysis, phytochemical screening, and bioactivities evaluation of Cissus rotundifolia (Forssk.) Vahl. (Fam. Vitaceae) and Sansevieria cylindrica Bojer ex Hook. (Fam. Dracaenaceae) growing in Egypt. Egypt Pharmaceut J 2015;14:180-6
|How to cite this URL:|
Said AA, Aboutabl EA, El Awdan SA, Raslan MA. Proximate analysis, phytochemical screening, and bioactivities evaluation of Cissus rotundifolia (Forssk.) Vahl. (Fam. Vitaceae) and Sansevieria cylindrica Bojer ex Hook. (Fam. Dracaenaceae) growing in Egypt. Egypt Pharmaceut J [serial online] 2015 [cited 2020 Dec 5];14:180-6. Available from: http://www.epj.eg.net/text.asp?2015/14/3/180/172864
| Introduction|| |
Cissus rotundifolia (Forssk.) Vahl. (Fam. Vitaceae) and Sansevieria cylindrica Bojer ex Hook. (Fam. Dracaenaceae) are native to the subtropical regions of the African continent, and cultivated in Egypt for ornamental purposes. Both plants are reputed to have beneficial effects in the traditional system of medicine. Cissus rotundifolia (Forssk.) Vahl. is a climber known as Arabian Wax Cissus or Peruvian grape ivy. It was reported to be used in Yemeni ethnomedicine to treat fever and digestive ailments . Certain Cissus spp. were reported to contain sterols, triterpenoids , phenolics , flavonoids, stilbene derivatives, iridoids , and coumarin glycosides . Different therapeutic effects were attributed to certain Cissus spp. . C. rotundifolia leaves showed antioxidant  and antibacterial activities .
On the other hand, S. cylindrica Bojer ex Hook., xerophytic perennial herb known as the Cylindrical Snake Plant, African Spear or Spear Sansevieria. Several steroidal sapogenins and saponins were isolated from certain Sansevieria spp. ,,,. A new steroidal saponin isolated from the leaves of S. cylindrica showed inhibition of the capillary permeability activity .
The main aim of the present study was to carry out a proximate analysis, phytochemical screening, and bioactivities evaluation of the two plants.
| Materials and methods|| |
The nonflowering aerial parts (stems and leaves) of each of C. rotundifolia (Fam. Vitaceae) and S. cylindrica (Fam. Dracaenaceae) were collected from Orman botanical garden, Giza, Egypt, in June 2009, kindly authenticated by Dr Mohammed El-Gebaly, Department of Botany, National Research Centre (NRC), and confirmed by Dr Abd El-Magali, Flora and Phytotaxonomy Research - Horticultural Research Institute, Agricultural Research Centre, Ministry of Agriculture. Voucher specimens are deposited in the Herbarium, Pharmacognosy Department, Faculty of Pharmacy, Cairo University.
Preparation of the methanolic extracts
The air-dried powdered nonflowering aerial parts (1 kg) of each plant were extracted by maceration with 70% aqueous methanol until exhaustion. The solvent was evaporated to dryness under reduced pressure to yield the crude extract of each plant.
Preparation of successive extracts
The powder of each plant (100 g) was extracted successively with petroleum ether, chloroform, ethyl acetate, and 70% methanol in a Soxhlet apparatus. Each extract was evaporated to dryness under reduced pressure. The solvent-free residue in each case was weighed.
Percentages of moisture content, total ash, water-soluble ash, acid-insoluble ash, and crude fiber values were assessed according to the official methods .
Preliminary phytochemical screening
All the extracts were screened qualitatively for the presence of various groups of phytoconstituents using different chemical tests ,.
Total phenolic assay
The total phenolic content was determined applying the Folin-Ciocalteu colorimetric method using gallic acid as a standard  and expressed as milligrams of gallic acid equivalents (GAE)/g of the dry plant material.
Total flavonoid assay
Total flavonoid content (TFC) was measured using an aluminum chloride colorimetric assay . A calibration curve was established using quercetin as a standard. TFC was expressed as mg quercetin equivalent (QE)/g of the dry plant material.
Adult male albino Sprague-Dawley rats weighing 130-150 g and adult male Swiss albino mice weighing 20-25 g were obtained from the animal house colony in the National Research Centre (Giza, Egypt). They were housed in groups in properly numbered stainless-steel cages, maintained on a 12 h light and dark cycle at 28 ± 2C in a well-ventilated animal house under natural conditions, and given pelleted food and drinking water ad libitum.
Acute oral toxicity study
Seventy albino rats were grouped randomly into one control group and six treated groups, each including ten animals. Animals were orally dosed 0.25, 0.5, and 1 g/kg body weight of the tested extracts following the method of Lorke . Control animals received the vehicle and were kept under the same conditions without any treatment. Animals were observed for 24 h for signs of toxicity or death.
For 3 consecutive days preceding the experiment, mice were adapted on the hot plate by placing them on a plate maintained at room temperature for 15 min each day. Each animal was then placed gently onto a 52C ( ± 0.1C) hot plate to perform the test. Six groups (six mice each) were administered vehicle and/or the tested extract of each plant (100 and 200 mg/kg body weight of extract) and the last group received indomethacin (10 mg/kg body weight). All drugs were injected subcutaneously 30 min before placing the animal on the hot plate. Latency to show nociceptive responses, such as licking paws or jumping off the hot plate, was determined 30, 60, and 120 min after administration of the test substances or saline .
Six separate groups (six mice each) were administered the vehicle and/or the tested extract (100 and 200 mg/kg body weight) subcutaneously 30 min before the intraperitoneal injection of 0.6% acetic acid in distilled water (10 ml/kg). The stretching reaction was observed . Each mouse was then placed in an individual clear plastic observational chamber. The number of writhes (muscular contractions) was counted for 30 min immediately after the acetic acid injection and expressed as writhing numbers .
The carrageenan-induced rat-paw edema
Paw edema was induced by a subplantar injection of 100 μl of 1% sterile carrageenan in saline into the right hind paw . Thirty-six adult male albino rats, divided into six groups, each of six animals, were orally treated with 100 or 200 mg/kg body weight of 70% methanolic extract of C. rotundifolia and S. cylindrica nonflowering aerial parts, indomethacin (10 mg/kg body weight) (positive control), and saline (negative control). The rats received vehicle or extracts 1 h before carrageenan injection. The hind paw volume was measured immediately before carrageenan injection and at selected times thereafter with the water displacement method  using 7410, plethysmometer (Ugo Basile, Comerio, Italy).
Indomethacin-induced ulcer in pyloric-ligated rats
Pyloric-ligated rats were treated according to the method described by Shay and colleagues ,. Rats were starved for 18 h, but allowed free access to drinking water. A midline ventral incision starting from the xiphoid cartilage downwards was made to expose the stomach and the duodenum. The pylorus was ligated and the abdominal wall was sutured. The tested extracts were administered immediately after pyloric ligation, followed by oral administration of indomethacin (30 mg/kg body weight). Four hours later, animals were killed by cervical dislocation, the abdominal cavity was opened, and a ligature was placed at the esophagocardiac junction and the stomach was removed. The mucosa was examined  and the total lesion number was counted and the severity of lesions was calculated on the basis of the following score: 0 = no ulcer, 1 = lesion≤1 mm, 2 = lesion of size 1-2 mm, 3 = lesion of size 2-3 mm, 4 = lesion of size 3-4 mm, and 5 = lesion of size more than 4 mm.
Evaluation of hepatoprotective activity
Thioacetamide-induced acute hepatic encephalopathy
Forty-two rats were used to carry out the study and they were divided into seven groups (six animals each) as follows.
Group 1 (control group): rats received saline (2 ml/kg body weight) intraperitoneally once, and were then administered saline and corn oil orally at a dose of 5 ml/kg body weight daily for 3 days.
Group 2 [hepatotoxic thioacetamide (TAA) group]: the rats received TAA (300 mg/kg body weight) intraperitoneally once .
Groups 3, 4, 5, and 6 (treated groups): rats received TAA (300 mg/kg body weight) once intraperitoneally on the first day and were then orally administered a 70% aqueous methanolic extract of C. rotundifolia or S. cylindrica nonflowering aerial parts at two dose levels (100 or 200 mg/kg body weight) daily for 3 days.
Group 7 (silymarin and TAA): the rats received TAA (300 mg/kg body weight) once intraperitoneally on the first day and were then administered silymarin (100 mg/kg body weight) daily for 3 days.
The animals received dextrose water and ringer lactate solutions (10 mg/kg/day, intraperitoneally) to prevent renal failure, hypoglycemia, and electrolyte imbalance until the end of the experiment. All rats were killed under anesthesia 24 h after the last treatment and overnight fasting. Blood samples (∼4 ml) were taken from each rat from retro-orbital veins, using a sterile syringe, after they were killed at the end of the experiments. These samples were kept at room temperature for 30 min and centrifuged at 3000 rpm for 10 min. Serum samples were stored in a freezer (−20C) for use in biochemical analyses.
Hepatic biochemical parameters in serum
Serum aspartate transaminase (AST) and alanine transaminase (ALT) were determined according to the method of Reitman and Frankel  using ELISA kits supplied by Bio-diagnostic Company (Cairo, Egypt).
Values were expressed as means ± SE. Data of carrageenan-induced rat-paw edema, the hot-plate test, and the writhing test were analyzed using repeated-measures two-way analysis of variance, followed by the Tukey HSD test for multiple comparisons. Results of ulcer numbers and severity were analyzed using the Kruskal-Wallis nonparametric test, followed by Dunn's multiple-comparisons test. Hepatic biochemical parameters were analyzed using one-way analysis of variance or the corresponding nonparametric (Kruskal-Wallis) test, as required. A probability level of less than 0.05 was considered significant in all types of statistical tests.
| Results and discussion|| |
Percentages of moisture content, total ash, water-soluble ash, acid-insoluble ash, and crude fiber of each of the two plants were estimated and the results are presented in [Table 1]. Successive extracts of each of the two plants are shown [Table 2] and [Table 3] as percentage of yield, physical characteristics, and phytoconstituents.
|Table 1: Pharmacopoeial constants of Cissus rotundifolia and Sansevieria cylindrica nonflowering aerial parts|
Click here to view
|Table 2: Percentages, physical characteristics, and constituents of successive extracts of Cissus rotundifolia nonfl owering aerial parts|
Click here to view
|Table 3: Percentages, physical characteristics, and constituents of successive extracts of Sansevieria cylindrica nonflowering aerial parts|
Click here to view
Phytochemical analysis of 70% methanolic extract of C. rotundifolia nonflowering aerial parts indicated the presence of triterpenes, carbohydrates, and/or glycosides, tannins, flavonoids, coumarins, and saponins, whereas the 70% methanolic extract of S. cylindrica nonflowering aerial parts contains sterols, carbohydrates, and/or glycosides, flavonoids, coumarins, and saponins [Table 2] and [Table 3].
Total phenolic and flavonoids content
Total phenolic contents of C. rotundifolia and S. cylindrica nonflowering aerial parts estimated using the Folin-Ciocalteu method were 3.18 and 4.82 mg (GAE)/g, respectively, whereas the TFC were 1.35 and 4.13 mg (QE)/g plant dry weight, respectively.
Acute oral toxicity
At all of the doses tested, none of the extracts produced any mortality up to 24 h observation. Accordingly, the doses used for pharmacological studies were (100 and 200 mg/kg body weight) of each of the two extracts. These doses represent 1/10 and 1/5 of the maximum soluble dose of the aqueous extract that induced no mortalities in mice.
The results presented in [Table 4] indicate that the mean reaction time on the hot plate was significantly delayed after the administration of the tested extracts compared with indomethacin as a positive control, indicating the significant central analgesic effects of the tested extracts.
|Table 4: Central analgesic effect of 70% methanolic extracts of nonflowering aerial parts of Cissus rotundifolia and Sansevieria cylindrica as shown by the hot-plate test|
Click here to view
The results presented in [Table 5] indicate that acetic acid-induced writhing was significantly reduced in mice receiving tested extracts at the two dose levels, compared with indomethacin as a positive control, confirming the peripheral analgesic effect.
|Table 5: Antinoeiceptive effect of 70% methanolic extracts of nonflowering aerial parts of Cissus rotundifolia and Sansevieria cylindrica as shown by the acetic acid-induced writhing test|
Click here to view
Carrageenan-induced rat-paw edema
Pretreatment with C. rotundifolia and S. cylindrica extracts significantly decreased carrageenan-induced edema [Table 6]. The highest anti-inflammatory potency at 4 h was shown by C. rotundifolia. Both dose levels showed percentages of edema inhibition higher than or equal to the reference drug indomethacin. However, S. cylindrica, at both doses, showed lower percentages of edema inhibition than the reference drug indomethacin.
|Table 6: Anti-inflammatory effect of 70% methanolic extracts of nonflowering aerial parts of Cissus rotundifolia and Sansevieria cylindrica at doses of 100 and 200 mg/kg body weight on carrageenan-induced rat-paw edema|
Click here to view
The number of lesions in the untreated ulcer group was 4.03 ± 0.79, with an ulcer severity value of 9.5 ± 1.78. The groups pretreated with both methanolic extracts had no lesions, indicating a significant protection against ulcer. The number of lesions present on the gastric mucosa is indicative of the gastric damage . The absence of lesions in the groups pretreated with plant extract, compared with the ranitidine pretreated group, can possibly be attributed to inhibition of gastric acid secretion, an important factor in ulcer, or inhibition of lipid peroxidation because of their antioxidant activities. Ranitidine, an antisecretory drug, has often been reported to possess antioxidant and immunosuppressive actions, which might be responsible for its antiulcerogenic activity .
Evaluation of hepatoprotective activity
In the TAA group, serum levels of AST and ALT were significantly higher than those of the healthy controls (P < 0.0001), indicating liver injury [Table 7]. Rats treated with silymarin (100 mg/kg body weight) showed a significant reduction in their plasma AST and ALT by 66.48 and 41.59%, respectively, compared with the TAA control group, indicating the hepatoprotective effect. Rats treated with the 70% aqueous methanolic extract of nonflowering aerial parts of C. rotundifolia at doses of 100 and 200 mg/kg body weight showed elevated levels of their ALT and AST, indicating that C. rotundifolia does not exert any hepatoprotective effect. However, rats treated with 70% aqueous methanolic extract of S. cylindrica nonflowering aerial parts at a dose of 100 mg/kg body weight showed a significant reduction in their plasma AST and ALT by 41 and 49.66%, respectively. Rats treated with a dose of 200 mg/kg body weight of S. cylindrica extract showed a significant reduction only in their plasma ALT by 27.56%, indicating its hepatoprotective effect.
|Table 7: Effect of 70% aqueous methanolic extract of nonflowering aerial parts of Cissus rotundifolia and Sansevieria cylindrica in thioacetamide-induced liver injury|
Click here to view
| Conclusion|| |
The different parameters developed in this study will enable phytochemical identification and standardization of both plants. The results of the preliminary phytochemical screening and bioactivities evaluation indicate that C. rotundifolia (Forssk.) Vahl. and S. cylindrica Bojer ex Hook can be potential sources of phytomedicines with analgesic, anti-inflammatory, and antiulcerative activities. Moreover, S. cylindrica is a potential source of a hepatoprotective phytomedicine.
The authors wish to acknowledge the financial support of the National Research Centre.
Financial support and sponsorship
Conflicts of interest
There are no conflicts of interest.
| References|| |
Al-Fatimi M, Wurster M, Schröder G, Lindequist U. Antioxidant, antimicrobial and cytotoxic activities of selected medicinal plants from Yemen. J Ethnopharmacol 2007; 111:657-666.
Pan GY, Li WF, Luo P, Qin JK, Su GF. Study on steroidal and triterpenoid constituents from Cissus pteroclada
. Zhong Yao Cai 2013; 36:1274-1277.
Quílez AM, Saenz MT, García MD, de la Puerta R. Phytochemical analysis and anti-allergic study of Agave intermixta
Trel. and Cissus sicyoides
L. J Pharm Pharmacol 2004; 56:1185-1189.
Singh G, Rawat P, Maurya R. Constituents of Cissus quadrangularis
. Nat Prod Res 2007; 21:522-528.
Beltrame FL, Ferreira AG, Cortez DA. Coumarin glycoside from Cissus sicyoides
. Nat Prod Lett 2002; 16:213-216.
Fernandes G, Banu J. Medicinal properties of plants from the genus Cissus
: a review. J Med Plants Res 2012; 6:3080-3086.
Al-Mamary MA Jr. Antioxidant activity of commonly consumed vegetables in Yemen. Malays J Nutr 2002; 8:179-189.
Gamboa-Angulo MM, Reyes-Lopez G, Pene-Rodriguez LM. A natural pregnane from Sansevieria hyacinthoides
. Phytochemistry 1996; 43:1079-1081.
Mimaki Y, Inoue T, Kuroda M, Sashida Y. Steroidal saponins from Sansevieria trifasciata
. Phytochemistry 1996; 43:1325-1331.
Mimaki Y, Inoue T, Kuroda M, Sashida Y. Pregnane glycosides from Sansevieria trifasciata
. Phytochemistry 1997; 44:107-111.
Pettit GR, Zhang Q, Pinilla V, Hoffmann H, Knight JC, Doubek DL, et al
. Antineoplastic agents. 534. isolation and structure of sansevistatins 1 and 2 from the African Sansevieria ehrenbergii
. J Nat Prod 2005; 68:729-733.
Da Silva Antunes A, Da Silva BP, Parente JP, Valente AP. A new bioactive steroidal saponin from Sansevieria cylindrica
. Phytother Res 2003; 17:179-182.
Egyptian Pharmacopoeia. General Organization for Government Printing Affairs. Cairo: Egyptian Pharmacopoeia; 2005.
Trease GE, Evans WC. Textbook of pharmacognosy
. 12th ed. London: Bailliere Tindall; 1989.
Harborne JB. Phytochemical methods - a guide to modern techniques of plant analysis. Chapman and Hall
. London; 1998.
Siger A, Nogala-Kalucka M, Lampart-Szczapa E. The content and antioxidant activity of phenolics compounds in cold-pressed plant oils. J Food Lipids 2008; 15:137-149.
Zhishen J, Mengcheng T, Jianming W. The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chem 1999; 64:555-559.
Lorke D. A new approach to practical acute toxicity testing. Arch Toxicol 1983; 54:275-287.
Laviola G, Alleva E. Ontogeny of muscimol effects on locomotor activity, habituation, and pain reactivity in mice. Psychopharmacology (Berl) 1990; 102:41-48.
Koster R, Anderson M, de Beer EJ. Acetic acid for analgesic screening. Feder Proc 1959; 18:412-416.
Chakraborty A, Devi RK, Rita S, Sharatchandra K, Singh TI. Preliminary studies on anti-inflammatory and analgesic activities of Spilanthes acmella
in experimental animal models. Indian J Pharmacol 2004; 36:148-150.
Winter CA, Risley EA, Nuss GW. Carrageenin-induced oedema in hind paw of the rat as an assay for antiinflammatory drugs. Proc Soc Exp Biol Med 1962; 111:544-547.
Chattopadhyay D, Arunachalam G, Mandal AB, Sur TK, Mandal SC, Bhattacharya, SK. Antimicrobial and anti-inflammatory activity of folklore: Mallotus peltatus
leaf extract. J Ethnopharmacol 2002; 82:229-237.
Shay H, Komarov SA, Fels SS, Meranze D, Gruenstein M, Siplet H. A simple method for the uniform production of gastric ulceration in the rat. Gastroenterology 1945; 5:43-61.
Abd El-Hady FK, El Awdan SA, Ibrahim AM. Anti-ulcerative potential of Egyptian propolis against oxidative gastric injury induced by indomethacin in rats. Asian J Med Pharm Res 2013; 3:35-42.
Mózsik G, Morón F, Jávor T. Cellular mechanisms of the development of gastric mucosal damage and of gastrocytoprotection induced by prostacyclin in rats. A pharmacological study. Prostaglandins Leukot Med 1982; 9:71-84.
Pawa S, Ali S. Liver necrosis and fulminant hepatic failure in rats: protection by oxyanionic form of tungsten. Biochim Biophys Acta 2004; 1688:210-222.
Reitman S, Frankel S. A colorimetric method for the determination of serum glutamic oxalacetic and glutamic pyruvic transaminases. Am J Clin Pathol 1957; 28:56-63.
West GB. Testing for drugs inhibiting the formation of gastric ulcers. J Pharmacol Methods 1982; 8:33-37.
Ardestani SK, Janlow MM, Kariminia A, Tavakoli Z. Effect of cimetidine and ranitidine on lipid profile and lipid peroxidation in γ-irradiated mice. Acta Med Iran 2004; 42:198-204.
[Table 1], [Table 2], [Table 3], [Table 4], [Table 5], [Table 6], [Table 7]