ORIGINAL ARTICLE
Year : 2015  |  Volume : 14  |  Issue : 3  |  Page : 180-186

Proximate analysis, phytochemical screening, and bioactivities evaluation of Cissus rotundifolia (Forssk.) Vahl. (Fam. Vitaceae) and Sansevieria cylindrica Bojer ex Hook. (Fam. Dracaenaceae) growing in Egypt


1 Department of Pharmacognosy, National Research Centre, Dokki, Giza, Egypt
2 Pharmacognosy Department, Faculty of Pharmacy, Cairo University, Cairo, Egypt
3 Department of Pharmacology, National Research Centre, Dokki, Giza, Egypt

Correspondence Address:
Mona A Raslan
PhD, Pharmacognosy Department, National Research Centre, Dokki, 12622 Giza
Egypt
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/1687-4315.172864

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Objectives This work aimed to carry out a proximate analysis, phytochemical screening, and bioactivities evaluation of the nonflowering aerial parts of each of Cissus rotundifolia (Forssk.) Vahl. (Fam. Vitaceae) and S. cylindrica Bojer ex Hook. (Fam. Dracaenaceae) growing in Egypt. Materials and methods The total phenolic contents (TPC) and total flavonoid contents were estimated spectrophotometrically. The oral acute toxicity of each of the two plant extracts was evaluated. Analgesic activities of both plants were evaluated using hot plate and writhing test using indomethacin as a reference drug. Anti-inflammatory activities were evaluated using carrageenan-induced rat-paw edema. Antiulcerative activities were measured using indomethacin-induced ulcer in pyloric-ligated rats. Thioacetamide-induced acute hepatic encephalopathy was used to evaluate the hepatoprotective effects of the plants. Results and conclusion Proximate analysis of air-dried nonflowering aerial parts of each of C. rotundifolia and S. cylindrica yielded a moisture content of 7.95 and 7.52%, total ash of 8.35 and 9.83%, water-soluble ash of 3.96 and 2.94%, acid-insoluble ash of 2.14 and 3.42%, crude fiber content of 8.52 and 13.43%, and methanolic extractive value of 41 and 35%, respectively. Phytochemical analysis of C. rotundifolia extract indicated the presence of triterpenes, carbohydrates, and/or glycosides, tannins, flavonoids, coumarins, and saponins, whereas S. cylindrica extract showed the presence of sterols, carbohydrates, and/or glycosides, flavonoids, coumarins, and saponins. Total phenolic content of C. rotundifolia and S. cylindrica were 3.18 and 4.82 mg (GAE)/g, respectively, whereas total flavonoid contents of C. rotundifolia and S. cylindrica were 1.35 and 4.13 mg (QE)/g plant dry weight, respectively. Both extracts showed no toxicity up to 1 g/kg body weight. Both methanolic extracts showed significant central and peripheral analgesic effects. C. rotundifolia extract showed percentages of edema inhibition higher than or equal to the reference drug indomethacin in carrageenan-induced paw edema assay, whereas S. cylindrica extract showed percentages of edema inhibition less than that of the reference drug indomethacin. In the ulcer model induced by indomethacin, the groups pretreated with both plants received significant protection against ulcer formation. In the thioacetamide-induced acute hepatopathic encephalopathy model, rats treated with S. extract showed significant improvement in their plasma AST and ALT, whereas rats treated with C. rotundifolia extract showed increased levels of their ALT and AST.


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