ORIGINAL ARTICLE
Year : 2016  |  Volume : 15  |  Issue : 1  |  Page : 38-42

Inhibition of human pathogenic bacteria by Moringa oleifera cultivated in Jazan (Kingdom of Saudi Arabia) and study of synergy to amoxicillin


Department of Biology, Faculty of Science, King Khalid University, Abha, Saudi Arabia; Department of Microbiology, National Organization for Drug Control and Research (NODCAR), Cairo, Egypt

Correspondence Address:
Mona Kilany
Department of Biology, Faculty of Science, King Khalid University, PO Box 9004, Abha, Saudi Arabia

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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/1687-4315.184029

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Background The spread of multidrug-resistant strains of bacteria to most antibiotics has led to intensive searches for effective strategies to overcome bacterial infections. Aim Moringa oleifera could be a potentially useful agent for many therapeutic applications, especially antimicrobial. Settings and design The leaves of M. oleifera were collected in October 2014 from Jazan, located in the southern region of the Kingdom of Saudi Arabia. Materials and methods Different organic solvents were used to extract antimicrobial substances in the plant leaves. The antibacterial activity of each leaf extract was determined in vitro and compared with some antibiotics. Minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) of both plant extracts and amoxicillin were investigated against Bacillus spp. An evaluation of the synergistic effects of amoxicillin and plant extract was carried out. Results The results showed that an ethanol extract is the best organic solvent for extracting the antimicrobial substance from M. oleifera. In addition, the antibacterial potential of an ethanol extract is comparable to that of some commercial antibiotics. MIC and MBC of the plant extract were 320 and 620 μg/ml, respectively, whereas MIC and MBC of amoxicillin were 25 and 50 μg/ml, respectively. The fractional inhibitory concentration of the plant extract and amoxicillin was determined to be 0.125 and 0.25, respectively. Therefore, ethanolic plant extract can be considered a good synergistic factor to amoxicillin, yielding a fractional inhibitory concentration index 0.375 ≤ 0.5 of the combination. Conclusion M. oleifera leaves may serve as a natural alternative to antibiotics. Moreover, M. oleifera can boost the inhibitory effect of amoxicillin, leading to a reduction of administration dose as well as minimizing the side effects of antibiotics.


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