ORIGINAL ARTICLE
Year : 2016  |  Volume : 15  |  Issue : 2  |  Page : 78-87

Effects of Roselle calyx (Hibiscus sabdariffa L.)-supplemented diets on growth and disease (Aeromonas hydrophila) resistance in Nile tilapia (Oreochromis niloticus L.)


1 Chemistry Department, Faculty of Science, Zagazig University, Zagazig, Egypt
2 Fish Nutrition Department, Central Lab for Aquaculture Research, Abassa, Abu Hammad, Sharkia, Egypt
3 Department of Natural Product, Division of Pharmaceutical Drug Industries, National Research Centre, Cairo (ID:60014618), Egypt
4 Central Lab for Agriculture Climate, Giza, Egypt

Correspondence Address:
Ahmad M. A. Souleman
Department of Natural Product, National Research Center, Division of Pharmaceutical Chemistry, Cairo
Egypt
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/1687-4315.190403

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Aims The aim of this study was to determine the effect of phenolic compounds of Roselle calyx (Hibiscus sabdariffa Linn) on growth performance, feed utilization, whole-body composition, blood profile, and immunity against Aeromonas hydrophila in Nile tilapia, Oreochromis niloticus. Background H. sabdariffa extracts have demonstrated to have a broad range of therapeutic effects such as antioxidant, antiobesity, anticancer, inhibition of the contractility of rat bladder and uterus, antibacterial, antihypertensive, and antimicrobial activities due to the presence of phenolic compounds. Materials and methods Fifteen fish with the same initial weight (5.45 ± 0.01 g/fish) were selected and randomly distributed into four experimental treatments in triplicates. The chemical profiles of phenolic constituents were analyzed using high-performance liquid chromatography to determine the major phenolic compounds. Results and discussion The diet containing 1% Roselle calyx showed the highest growth performance and feed utilization in comparison with other dietary treatments. The main phenolic compounds were ferulic acid, rosmarinic acid, apigenin, carnosic acid, cinnamic acid, caffeic acid, chlorogenic acid, p-Coumaric acid, and quercetin-7-O-glucoside, in addition to anthocyanins such as delphinidin-3-glucoside, sambubioside, cyanidin-3-monoglucoside, and cyanidin-3-sambubioside. Conclusion Roselle calyx was found to have an antibacterial activity antagonistic to pathogenic A. hydrophila infection in fish. Lysozyme and bactericidal activities of fish increased significantly in fish fed on Roselle calyx-enriched diets.


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