ORIGINAL ARTICLE
Year : 2019  |  Volume : 18  |  Issue : 1  |  Page : 42-52

Production, characterization, and antioxidant activities of bacterial exopolysaccharides extracted from petroleum oil water


1 Department of Microbial Biotechnology, National Research Centre, Dokki, Giza, Egypt
2 Department of Aromatic Plants, National Research Centre, Dokki, Giza, Egypt

Correspondence Address:
Manal S Selim
Department of Microbial Biotechnology, National Research Centre, 12311 Dokki, Giza
Egypt
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/epj.epj_36_18

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Backgroundand objective There are large quantities of water produced during the extraction of petroleum oil called ‘produced water’. The aim of this research is to isolate and identify bacteria produced exopolysaccharides (EPSs) present in this water and to evaluate their antioxidant activities as medicinal value. Materials and methods First isolation of Fe, Mn, and biofilm bacteria, next production of of EPSs, then morphological, physiological, and molecular characterization of isolates produced EPSs. Finally, study the characters of EPSs chemically and evaluate the antioxidant activity of EPSs by DPPH (1, 1-diphenyl-2-picrylhydrazyl) radical-scavenging model, reducing power, and by superoxide anion-scavenging activity. Results and conclusion Three bacteria were isolated from water of petroleum oil (produced water).These isolates produced water-soluble EPSs called Fe, Mn, and BF-EPSs and the highest production were 4.5, 7.5, and 5 g/l, respectively. The isolates were identified as Bacillus subtilis SMM1, Bacillus pumilus SMM2, and Bacillus tequilensis SMM3. Results showed that the three EPSs were acidic with different compositions of monosaccharide and different molar ratio. Uronic acid and SO3 were estimated. EPSs scavenged superoxide radical (O2−), DPPH radicals, and reducing power property. Fe-EPS was the most effective one in scavenging the superoxide radical and DPPH radicals while the highest reductant is BF-EPS. The obtained results demonstrated that all EPSs that have strong antioxidant activity can be used in medicinal and nutritional applications related to reduction of oxidative stress.


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