GC-MS analysis and in-vitro hypocholesterolemic, anti-rotavirus, anti-human colon carcinoma activities of the crude extract of a Japanese Ganoderma spp
Waill A Elkhateeb1, Ghoson M Daba1, Donia Sheir1, Asmaa Negm El-Dein1, Walid Fayad2, ELmahdy M Elmahdy3, Mohamed N.F Shaheen3, Paul W Thomas4, Ting-Chi Wen5
1 Chemistry of Natural and Microbial Products Department, Pharmaceutical Industries Researches Division, National Research Centre, Dokki, Egypt
2 Drug Bioassay-Cell Culture Laboratory, Pharmacognosy Department, National Research Centre, Dokki, Egypt
3 Environmental Virology Laboratory, Water Pollution Research Department, Environmental Research Division, National Research Centre, Dokki, Egypt
4 Mycorrhizal Systems Ltd, Lancashire; University of Stirling, Stirling, UK
5 The Engineering Research Center of Southwest Bio-Pharmaceutical Resources, Ministry of Education, Guizhou University, Guiyang, China
Ghoson M Daba
Chemistry of Na tural and Microbial Products Department, National Research Centre, Dokki, Giza 12622
Source of Support: None, Conflict of Interest: None
Background and objective Medicinal mushrooms are mines of various biologically active compounds. Therefore, chemical analysis and in-vitro evaluation of some biological activities of the Japanese originated mushroom Ganoderma spp. were conducted.
Materials and methods Extraction of the fruiting bodies of Ganoderma spp. was accomplished using 80% methanol. This extract was investigated for its in-vitro cholesterol-lowering activity, anti-rotavirus effect, and anti-human colon cancer influence. Moreover, a gas chromatography–mass spectrometry analysis for this extract was performed.
Results and conclusion The gas chromatography–mass spectrometry analysis resulted in the detection of 39 compounds, which were generally saturated and unsaturated fatty acids, and alkenes. The crude extract exhibited a promising in-vitro cholesterol-lowering activity (100±0%) after 96 h of incubation at room temperature. The same crude extract showed a moderate anti-rotavirus SA-11 strain effect with a therapeutic index of 9.3. Moreover, Ganoderma spp. extract displayed a strong activity toward HCT116 human colon carcinoma cell line, resulting in a cytotoxicity of 84.03±0.93% on HCT116 cell line monolayers. Ganoderma spp. crude extract represents a promising source of biologically active compounds that could by further investigations represent support and/or alternative to the currently used drugs.