Egyptian Pharmaceutical Journal

ORIGINAL ARTICLE
Year
: 2015  |  Volume : 14  |  Issue : 1  |  Page : 10--14

Estimation of total phenolic, tannins, and flavonoid contents and antioxidant activity of Cedrus deodara heart wood extracts


Sourabh Jain1, Aakanchha Jain2, Sanjay Jain3, Neelesh Malviya3, Vikas Jain4, Dharmendar Kumar5 
1 Smriti College of Pharmaceutical Education, Indore; Bhagyoday Tirth Pharmacy College, Sagar; CARPC, Shobhit University, Meerut, Uttar Pradesh, India
2 Department of Pharmaceutical Science, Dr H.S. Gour University, Sagar, India
3 Smriti College of Pharmaceutical Education, Indore, India
4 Mahakal Institute of Pharmaceutical Studies, Ujjain; CARPC, Shobhit University, Meerut, Uttar Pradesh, India
5 CARPC, Shobhit University, Meerut; Laureate Institute of Pharmacy, Himachal Pradesh, India

Correspondence Address:
Sourabh Jain
Centre for Advanced Research in Pharmaceutical Science, Shobhit Uinversity, Meerut, Uttar Pradesh
India

Purpose The present study was investigated to determine in-vitro antioxidant activity and total phenolic, total flavonoids, and total tannins contents of extracts of Cedrus deodara heart wood. Materials and methods Antioxidant activity of aqueous and alcoholic extract of C. deodara heart wood was evaluated against 2,2-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), 1,1-diphenyl-2-picryl hydrazyl (DPPH), and hydroxyl radical-scavenging activity models. Total flavonoids, tannins, and phenolic content of C. deodara were also determined. Results and conclusion Among both extracts, aqueous extract showed the highest total phenolic contents (23.97 μg/g of gallic acid equivalent/g of extract). In DPPH, superoxide anion, and ABTS scavenging test, the IC 50 (μg/ml) value of aqueous and alcoholic extract was 61.89, 75.79, 87.76, 121.55, 115.29, and 122.42, respectively. Hence, the above evidences suggest that C. deodara heart wood is a potential source of natural antioxidant and can be used to prevent diseases associated with free radicals.


How to cite this article:
Jain S, Jain A, Jain S, Malviya N, Jain V, Kumar D. Estimation of total phenolic, tannins, and flavonoid contents and antioxidant activity of Cedrus deodara heart wood extracts.Egypt Pharmaceut J 2015;14:10-14


How to cite this URL:
Jain S, Jain A, Jain S, Malviya N, Jain V, Kumar D. Estimation of total phenolic, tannins, and flavonoid contents and antioxidant activity of Cedrus deodara heart wood extracts. Egypt Pharmaceut J [serial online] 2015 [cited 2020 Mar 29 ];14:10-14
Available from: http://www.epj.eg.net/article.asp?issn=1687-4315;year=2015;volume=14;issue=1;spage=10;epage=14;aulast=Jain;type=0