Egyptian Pharmaceutical Journal

: 2018  |  Volume : 17  |  Issue : 2  |  Page : 77--84

Evaluation of an inhibitory effect of edible mushroom extracts against rotavirus infection

Abdou K Allayeh1, Reham M Elbaz2, Mohamed O Osman2, Norehan M Saeed3,  
1 Water Pollution Department, National Research Centre, Cairo, Egypt
2 Botany and Microbiology Department, Faculty of Science, Helwan University, Helwan, Egypt
3 Regional Centre for Blood Transfusion, Cairo, Egypt

Correspondence Address:
Abdou K Allayeh
Water Pollution Research Department, Environmental Research Division, National Research Centre, 33 El Bohouth Street, 12622 Dokki, Cairo


Background According to recent statistics by the WHO, the annual mortality rate associated with diarrhea is 30 deaths per 100 000 among Egyptian children younger than 5 years. Nearly 3.9% of the reported deaths are because of rotavirus infection. It is necessary to look for alternative treatment against rotavirus. Aim and methods The aim of this study is to investigate the antiviral activity of aqueous (AqE) and ethanol (EtOHE) extracts of the fruiting bodies of Agaricus spp and Pleurotus ostreatus against rotavirus infection using cell culture-based MTT assay. Results The tested extracts demonstrated significant inhibition effects against rotavirus infection up to 96.7, 90.6, 86.3, and 83.1% at concentration of 1000 μg/ml of P. ostreatus EtOHE, P. ostreatus AqE, Agaricus spp EtOHE, and Agaricus spp AqE, respectively, when added at zero time of the infection. Nothing was observed when extracts were added after viral infection. The synergistic activity was observed when different extracts were combined. Our results exhibited an inhibitory effect against different phases of rotavirus infection. Conclusion The use of edible mushrooms as a potential antiviral substance might be an alternative treatment against rotavirus infection. Nonetheless, more investigations are requiring for studying the efficacy in vivo and for segregating their fractions, which might clarify the mechanism of the inhibitory effect.

How to cite this article:
Allayeh AK, Elbaz RM, Osman MO, Saeed NM. Evaluation of an inhibitory effect of edible mushroom extracts against rotavirus infection.Egypt Pharmaceut J 2018;17:77-84

How to cite this URL:
Allayeh AK, Elbaz RM, Osman MO, Saeed NM. Evaluation of an inhibitory effect of edible mushroom extracts against rotavirus infection. Egypt Pharmaceut J [serial online] 2018 [cited 2020 Mar 31 ];17:77-84
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Rotaviruses are a major cause of acute gastroenteritis in children worldwide [1]. To date, there are two rotavirus vaccines (Rotarix and RotaTeq). Their effectiveness requires more efforts to develop and improve [2]. However, rotavirus vaccines are costly and not recommended to use in immunocompromised children. There is urgent need to look for alternative treatment against rotavirus infections. Natural products are one of the best candidates for antiviral drugs as they are effective and inexpensive. Currently, many natural compounds have known to have antirotavirus effects in clinical studies [3],[4],[5], in animal experiments [6], and in vitro [7],[8],[9],[10].

Edible mushrooms have been consumed for centuries as food and in a type of tea. It could be viewed as an extraordinary hotspot for proteins, carbohydrates, and bioactive metabolites for health promotion and disease prevention [11]. The previous studies suggested that mushrooms have been used as anticarcinogenic, antioxidant, or for stimulating the immune system. The therapeutic properties of mushrooms are mainly attributed to their content of polysaccharides and phenolic compounds, which exhibit anticancer, antiviral, antibacterial, and antioxidants activities [12],[13],[14],[15],[16],[17],[18].

The antiviral activity of edible mushroom has been demonstrated against HIV and western equine encephalitis virus (WEE), but there is nothing about its activity against rotavirus, particularly with respect to Agaricus spp. and Pleurotus ostreatus. For example, Wang and Ng [19] isolated a glycoprotein from fruiting body of P. ostreatus and demonstrated its antiviral activity against HIV. The previous report of Sorimachi et al. [20] showed that the aqueous extract of Agaricus brasiliensis has an inhibitory effect against WEE virus.

Generally, the antiviral activity of the compounds isolated from the consumable mushroom might be attributed to direct effect against viral infection or indirectly by stimulate the immune system [14],[21].

 Materials and methods

Sample preparation

Both aqueous and ethanol extracts of P. ostreatus and Agaricus spp. were kindly provided by Dr Heba E. El-Henawy, Botany and Microbiology Department, Faculty of Science, Helwan University, Egypt. In summary, to obtain the extracts, two grams of the fruiting bodies of each mushroom were dissolved in 10 ml of water or absolute ethanol at ambient temperature under agitation for 1 h. Extracts were filtered using 0.2-µm syringe filter (Millipore Corp., Bedford, Massachusetts, USA).

Cells and viruses

Rotavirus SA-11 strain was obtained from Virology Department of National Institute for Cholera and Enteric Diseases, Kolkata, India, by Dr Mohamed Nasr, National Research Centre. For rotavirus activation, 10 μg/ml of treated trypsin was added to virus suspension followed by incubation time for 60 min at 37°C. MA-104 (African green monkey kidney) cell line within the sight of 10 μg/ml of treated trypsin was used for rotavirus propagation. Use of treated trypsin was for increasing the infectivity of rotavirus [22],[23]. The virus titer was estimated using the limit-dilution method and expressed as a 50% cell culture infective dose of 1×106 [24]. Virus stock solution was stored at −80°C until use.

Determination of cytotoxicity by morphological changes

Cytotoxicity of the prepared extracts was estimated on uninfected MA-104 cell monolayers, which was prepared in 24-well plates containing Dulbecco’s modification of Eagle’s medium with 10% fetal bovine serum (FBS, Gibco BRL) and 100 μg/ml of antibiotic mixture. After 24 h of incubation at 37°C in atmosphere 5% CO2, the growth medium was discarded and 100 µl of various concentrations (1000, 500, 250, 125, and 0 μg/ml) of each extract were added to confluent MA-104 cell monolayers and incubated in fresh growth medium. After 24h and 48h of incubation, the morphological changes of examined cells were evaluated using inverted microscope.

Determination of cytotoxicity by MTT assay

According to Miranda et al. [25], 100 µl of 2×104 uninfected cells/well was seeded into 96-well plates and incubated for 24 h. The growth medium was removed and inoculated by various concentrations (1000, 500, 250, 125, and 0 μg/ml) in fresh medium for additional 48 h of incubation time at 37°C under humidified atmosphere of 5% CO2. The medium was replaced by 100 µl of MTT solution (5 mg/ml) and was incubated for further 4 h at 37°C. The MTT solution was replaced by 50 µl of acidified isopropanol and incubated for 30 min at 37°C. The optical density was identified using ELISA reader at 570 nm. The 50% cytotoxic concentration (CC50) was calculated as (A−B/A)×100, where A and B are equivalent to the mean of three OD570 of untreated and treated cells, respectively.

Determination of antiviral assay by MTT assay

One hundred microliter of MA-104 cells was cultured in 96-well plate, with a total number of 2×104 cells/well, and incubated for 24 h. The culture medium was replaced by adding 100 µl of tested extract or virus, according to the next sections of distinctive methodologies. A 50% inhibitory concentration (IC50) value was calculated as [(A−B)/(C−B)×100], where A, B, and C refer to the mean three absorbance of the tested extract with virus, virus positive control, and cell negative control, respectively, by using MTT assay. In addition, the therapeutic index has been calculated and characterized as the ratio of CC50/IC50.

The effect of addition time of each extract against CPE of rotavirus infection was determined according to Yang et al. [26]. Briefly, MA-104 cells cultured in 96 microplates were submitted into each concentration of the extract before (−1 and −2 h), zero time, and after (1 and 2 h) viral infection ([Figure 1]). After incubation, the antiviral activity was calculated using MTT assay, and each experiment was done in duplicates. (a) Postinfection assay was done as follows: 100 µl of MA-104 cells (2×104 cells/well) was cultivated in 96-well plate and incubated for 24 h. The culture medium was replaced by 100 µl of rotavirus SA-11 and incubated on the cells for 1 and 2 h at 37°C in 5% CO2. After incubation time, 100 µl of extract was added, followed by fresh growth medium and incubated for further 48 h. (b) For the zero time assay, the same previous procedures were performed, except that the 100 µl of each extract was added directly after inoculation of rotavirus without any incubation time. (c) Preinfection assay was done according to Zhu et al. [27], where 100 µl of MA-104 cells was cultivated and incubated for 24 h. The medium was replaced by 100 µl of extract, followed by incubation time for 1 or 2 h at 37°C in 5% CO2. Overall, 100 µl of rotavirus SA-11 was added to treated cells, followed by incubation time for 48 h. (d) For virucidal assay, 100 µl of both rotavirus and each concentration of extract was mixed together before inoculating into the cells, followed by 48 h of incubation.{Figure 1}

Synergistic activity

Synergy effect, combined activity of two different extracts, was investigated. Separate concentrations (125 and 250 μg/ml), which was examined previously during zero time assay, were combined and tested in the half quantity used during zero time assay. Herein, 50 µl of extract at concentration of 125 or 250 μg/ml was combined with 50 µl of different extract at the same concentration. Overall, 100 µl of extract mixture was added directly after inoculation of rotavirus followed by 48 h of incubation.

Confirmation of rotavirus infection

Viral RNA was extracted from MA-104 cells by Qiagen Kit (Hilden, Germany), according to the manufacturer’s instructions. Overall, 5 µl of extracted RNA was shock treated at 95°C for 5 min and chilled on ice for 5 min. A reverse transcription (RT)-PCR method based on amplification of a VP6 fragment was used. Rota-A forward primer: 5-GGATGTCCTGTACTCCTTGTCAAAA-3, and Rota-A reverse primer: 5-TCCAGTTTGGAACTCATTTCCA-3, each at a concentration of 1 µmol/l, were used in an RT reaction. Five microliter of cDNA product has been used for a PCR program, which was as 10 min at 95°C and 30 cycles of 15 s at 95°C, 1 min at 60°C, and 1 min at 72°C, which amplify a 144-bp product [28].


Cytotoxicity assay

In this work, the tested extracts of edible mushrooms (Agaricus spp. and P. ostreatus) have been investigated to study the inhibitory activity against rotavirus infection. The cytotoxicity was identified by using (a) optical microscopy for examination of the morphological changes of the treated cells and contrasted with untreated cells as a control. (b) Cytotoxicity was evaluated using MTT assay to calculate the CC50. No concentration of both extracts was poisonous at 1000 μg/ml. The CC50 for the AqE and EtOHE of Agaricus spp. was 2902 and 2525 μg/ml, respectively, whereas the CC50 was 6447 and 3550 μg/ml for the AqE and EtOHE of P. ostreatus, respectively ([Table 1]).{Table 1}

Effects of time of addition

Our outcomes demonstrated that the strongest percentages of viral inhibition were 96.7, 90.6, 86.3, and 83.1% at concentration of 1000 μg/ml of EtOHE of P. ostreatus, AqE of P. ostreatus, EtOHE of Agaricus spp., and AqE of Agaricus spp., respectively, when added at zero time. During virucidal mechanism of an infection, the tested extract (1000 μg/ml) showed moderate direct effect of viral inhibitions, which were 71, 56.5, 50.3, and 22% for EtOHE of P. ostreatus, AqE of P. ostreatus, EtOHE of Agaricus spp., and AqE of Agaricus spp., respectively, whereas in the preinfection period, the tested extracts showed little effect of viral inhibitions when added before viral infection, which were 27.9, 26.2, 41.0, and 20.0% for EtOHE of P. ostreatus, AqE of P. ostreatus, EtOHE of Agaricus spp., and AqE of Agaricus spp., respectively ([Figure 2],[Figure 3],[Figure 4],[Figure 5]). Finally, no inhibitory effect was observed when extracts were added after viral infection. The IC50 and the therapeutic index were identified depending on the outcomes of time of addition obtained at zero time ([Table 1]).{Figure 2}{Figure 3}{Figure 4}{Figure 5}

Synergistic effect between two mushroom extracts

Two mixed mushroom extracts demonstrated synergistic activity in this study. The AqE of Agaricus spp. (125 μg/ml) and EtOHE of P. ostreatus (125 μg/ml) inhibited viral infection with 19.0 and 51.1%, respectively, whereas a combination of both extracts of AqE of Agaricus spp. and EtOHE of P. ostreatus demonstrated an inhibition of up to 68.7%. In addition, the same mechanism of the synergistic effect was observed when EtOHE of Agaricus spp. and EtOHE of P. ostreatus have been combined, with an inhibition activity up to 77.0%. However, each single extract showed antiviral activity up to 16.4% with EtOHE of Agaricus spp. (125 μg/ml) and 51.1% with EtOHE of P. ostreatus (125 μg/ml) ([Figure 6] and [Figure 7]).{Figure 6} {Figure 7}


The measurement of 50% cell culture infective dose of simian rotavirus SA-11 strain was computed according to the Reed and Muench method [24]. In addition, the IC50 values were identified by statistically validated nonlinear regression curve and calculated from the mathematical regression curve formula.


Recently, there is an evolving eagerness for associating pharmacology with nourishment science. Pharmaceuticals are created to cure sickness, and the essential objective of nourishment is to keep up or enhance well-being. This does not propose that there is no part for nourishment in anticipating or curing affliction [29]. The extracts of consumable mushrooms have been investigated previously for their antioxidants, antibacterial, or antiviral effects, but nothing was reported about their antiviral activity against rotavirus [12],[13],[30],[31]. In this work, the antiviral activity of aqueous (AqE) and ethanol (EtOHE) extracts of Agaricus spp. and P. ostreatus has been investigated against CPE of rotavirus infection.

These extracts demonstrated a capability to inhibit the cytopathic effect of rotavirus infection depending on the concentration of the extract and the contact time. Both the AqE and the EtOHE extracts have solid inhibitory actions when added at zero time of viral infection, and moderate to little effects during virucidal and preinfection stages at concentration of 1000 μg/ml. Our findings are similar with the previous reports of Zhang et al. [32] and Faccin et al. [33]. They demonstrated that the fractions obtained from B-glycan isolated from P. tuberregium were more effective when added simultaneously with herpes simplex type 1 and 2 at the time of viral infection. In addition, the findings of virucidal and preinfection stages are similar with the results of Sorimachi et al. [20] and McClure et al. [34] They demonstrated that the fractions isolated from A. brasiliensis and Ganoderma lucidum mycelia slightly inhibited the early steps of viral replication.

Our results recommend a direct action of the extracts on viral replication and viral molecule itself, which prompted to repress the replication and adsorption stages. However, the outcomes of the present study showed no efficacy during postinfection phase in contrast with other phases of the viral infection. These results are similar with the previous reports of Liu et al. [21] and Lopes et al. [35]. They observed a remarkable antiviral activity during pretreated and treated herpes virus infection more than the treatment of postinfection.

The present study demonstrated that the effect of ethanol extracts was more valuable than aqueous extracts against rotavirus. These results were in agreement with the report of Sorimachi et al. [20], who studied the antiviral activity of ethanolic extract of Agaricus blazei against WEE virus, poliovirus, and herpes simplex virus. He found higher antiviral activity for ethanol extract more than aqueous extract.

The findings of this study showed that each extract alone have a potent inhibitory activity against rotavirus infection, but low concentrations have a weak effect. The combination between EtOHE of Agaricus spp. and EtOHE of P. ostreatus greatly inhibited rotavirus, which tended to be further decreased when compared with that of EtOHE of Agaricus spp. or EtOHE of P. ostreatus alone. These results suggested that EtOHE of Agaricus spp. and EtOHE of P. ostreatus have synergistic effect. This kind of combined treatment will offer an excellent method for treating severe diseases like those caused by viruses, because this kind of treatment would be benefit to inhibit the viral infection and decrease the toxicity [36].


The utilization of mushroom extracts as a possible element for the prevention or cure rotavirus infection might be an alternative treatment. These extracts might act on the viral particle and the replication cycle of rotavirus. However, more studies are required for studying the isolated fractions of these extracts that might clarify the mechanism of the antiviral activity.

Financial support and sponsorship


Conflicts of interest

There is no conflict of interest.


1Junaid SA, Umeh C, Olabode AO, Banda JM. Incidence of rotavirus infection in children with gastroenteritis attending Jos University Teaching Hospital, Nigeria. Virol J 2011; 8:233.
2Jiang V, Jiang B, Tate J, Parashar UD, Patel MM. Performance of rotavirus vaccines in developed and developing countries. Hum Vaccin 2010; 6:532–542.
3Vanderhoof J, Murray ND, Paule CL, Ostrom KM. Use of soy fiber in acute diarrhea in infants and toddlers. Clin Pediatr 1997; 36:135–139.
4Rabbani GH, Teka T, Zaman B, Majid N, Khatun M, Fuchs GJ. Clinical studies in persistent diarrhea: dietary management with green banana or pectin in Bangladeshi children. Gastroenterology 2001; 121:554–560.
5Subbotina MD, Timchenko VN, Vorobyov MM, Konunova YS, Aleksandrovih YS, Shushunov S. Effect of oral administration of tormentil root extract (Potentilla tormentilla) on rotavirus diarrhea in children: a randomized, double blind, controlled trial. Pediatr Infect Dis J. 2003; 22:706–711.
6Tam KI, Roner MR. Characterization of in vivo anti-rotavirus activities of saponin extracts from Quillaja saponaria Molina. Antiviral Res 2011; 90:231–241.
7Clark K, Grant P, Sarr A, Belakere J, Swaggerty C, Phillips T, Woode G. An in vitro study of theaflavins extracted from black tea to neutralize bovine rotavirus and bovine coronavirus infections. Vet Microbiol 1998; 63:147–157.
8Takahashi K, Matsuda M, Ohashi K, Taniguchi K, Nakagomi O, Abe Y et al. Analysis of anti-rotavirus activity of extract from Stevia rebaudiana. Antiviral Res 2001; 49:15–24.
9Andres A, Donovan SM, Kuhlenschmidt TB, Kuhlenschmidt MS. Isoflavones at concentrations present in soy infant formula inhibit rotavirus infection in vitro. J Nutr. 2007; 137:2068–2073.
10Lipson S, Sethi L, Cohen P, Gordon R, Tan I, Burdowski A, Stotzky G. Antiviral effects on bacteriophages and rotavirus by cranberry juice. Phytomedicine 2007; 14:23–30.
11Bishop KS, Kao CH, Xu Y, Glucina MP, Paterson RRM, Ferguson LR. From 2000 years of Ganoderma lucidum to recent developments in nutraceuticals. Phytochemistry 2015; 114:56–65.
12Menoli RCRN, Mantovani MS, Ribeiro LR, Speit G, Jordao BQ. Antimutagenic effects of the mushroom Agaricus blazei Murrill extracts on V79 cells. Mutat Res 2001; 496:5–13.
13Nakajima A, Ishida T, Koga M, Takeuchi T, Mazda O, Takeuchi M. Effect of hot water extract from Agaricus blazei Murill on antibody-producing cells in mice. Int Immunopharmacol 2002; 2:1205–1211.
14Lindequist U, Niedermeyer TH, Jülich WD. The pharmacological potential of mushrooms. Evid Based Complement Alternat Med 2005; 2:285–299.
15Huang Q, Jin Y, Zhang L, Cheung PC, Kennedy JF. Structure, molecular size and antitumor activities of polysaccharides from Poria cocos mycelia produced in fermenter. Carbohydr Polym 2007; 70:324–333.
16Gern RMM, Wisbeck E, Rampinelli JR, Ninow JL, Furlan SA. Alternative medium for production of Pleurotus ostreatus biomass and potential antitumor polysaccharides. Bioresour Technol 2008; 99:76–82.
17Aida F, Shuhaimi M, Yazid M, Maaruf A. Mushroom as a potential source of prebiotics: a review. Trends Food Sci Technol 2009; 20:567–575.
18Papaspyridi LM, Katapodis P, Gonou Zagou Z, Kapsanaki Gotsi E, Christakopoulos P. Optimization of biomass production with enhanced glucan and dietary fibres content by Pleurotus ostreatus ATHUM 4438 under submerged culture. Biochem Eng J. 2010; 50:131–138.
19Wang H, Ng T. Isolation of a novel ubiquitin like protein from Pleurotus ostreatus mushroom with antihuman immunodeficiency virus, translation inhibitory, and ribonuclease activities. Biochem Biophys Res Commun 2000; 276:587–593.
20Sorimachi K, Ikehara Y, Maezato G, Okubo A, Yamazaki S, Akimoto K, Akira N. Inhibition by Agaricus blazei Murill fractions of cytopathic effect induced by western equine encephalitis (WEE) virus on VERO cells in vitro. Biosci Biotechnol Biochem 2001; 65:1645–1647.
21Liu J, Yang F, Ye LB, Yang XJ, Timani KA, Zheng Y, Wang YH. Possible mode of action of antiherpetic activities of a proteoglycan isolated from the mycelia of Ganoderma lucidum in vitro. J Ethnopharmacol 2004; 95:265–272.
22Almeida JD, Hall T, Banatvala J, Totterdell B, Chrystie I. The effect of trypsin on the growth of rotavirus. J Gen Virol 1978; 40:213–218.
23Arias CF, Romero P, Alvarez V, Lopez S. Trypsin activation pathway of rotavirus infectivity. J Virol 1996; 70:5832–5839.
24Reed LJ, Muench H. A simple method of estimating fifty percent endpoints. Am J Epidemiol 1938; 27:493–497.
25Miranda M, Almeida A, Costa S, Santos M, Lagrota M, Wigg M. In vitro activity of extracts of Persea americana leaves on acyclovir resistant and phosphonoacetic resistant herpes simplex virus. Phytomedicine 1997; 4:347–352.
26Yang CM, Cheng HY, Lin TC, Chiang LC, Lin CC. Acetone, ethanol and methanol extracts of Phyllanthus urinaria inhibit HSV-2 infection in vitro. Antiviral Res 2005; 67:24–30.
27Zhu W, Chiu L, Ooi V, Chan P, Ang P. Antiviral property and mode of action of a sulphated polysaccharide from Sargassum patens against herpes simplex virus type 2. Int J Antimicrob Agents 2004; 24:279–283.
28Logan C, OLeary JJ, OSullivan N. Real time reverse transcription PCR for detection of rotavirus and adenovirus as causative agents of acute viral gastroenteritis in children. J Clin Microbiol 2006; 44:3189–3195.
29Georgiou NA, Garssen J, Witkamp RF. Pharmanutrition interface: the gap is narrowing. Eur J Pharmacol 2011; 651:1–8.
30Delmanto RD, de Lima PL, Sugui MM, da Eira AF, Salvadori DM, Speit G, Ribeiro LR. Antimutagenic effect of Agaricus blazei Murrill mushroom on the genotoxicity induced by cyclophosphamide. Mutat Res 2001; 496:15–21.
31Guterrez Z, Mantovani M, Eira A, Ribeiro L, Jordao B. Variation of the antimutagenicity effects of water extracts of Agaricus blazei Murrill in vitro. Toxicol In Vitro 2004; 18:301–309.
32Zhang M, Cheung PC, Ooi VE, Zhang L. Evaluation of sulfated fungal β-glucans from the sclerotium of Pleurotus tuber regium as a potential water soluble antiviral agent. Carbohydr Res 2004; 339:2297–2301.
33Faccin L, Benati F, Rincao V, Mantovani M, Soares S, Gonzaga M et al. Antiviral activity of aqueous and ethanol extracts and of an isolated polysaccharide from Agaricus brasiliensis against poliovirus type 1. Lett Appl Microbiol 2007; 45:24–28
34McClure MO, Moore JP, Blanco DF, Scotting P, Cook GM, Keynes RJ et al. Investigations into the mechanism by which sulfated polysaccharides inhibit HIV infection in vitro. AIDS Res Hum Retroviruses 1992; 8:19–26.
35Lopes N, Faccin-Galhardia L, Espadaa SF, Pacheco AC, Ricardo NM, Linhares RE, Nozawa C. Sulfated polysaccharides of Caesalpinia ferrea inhibits herpes simplex virus and poliovirus. Int J Biol Macromol 2013; 60:93–99.
36Yang T, Jia M, Yuea Z, Cheng Y, Zhang X, Huang J et al. Synergistic antivirus effect of combined administration of Combivir with Angelica polysaccharide sulfate. Int J Biol Macromol 2013; 53:122–126.