Figure 6: Effect of 6-aryl-9-substituted-6,9-diazaspiro-[4,5]decane-8,10- diones series 1– 9 on percentage protection in carrageenan-induced paw edema. Mice were injected with compounds 1– 9 (25 mg/kg, intraperitoneal) or diclofenac sodium (D) (10 mg/kg, intraperitoneal) or vehicle (C) 1 h before carrageenan challenge. The mouse hind paw was injected with 0.05 ml of 0.5% suspension of carrageenan in saline into the plantar tissue. The other hind paw was injected with an equal volume of saline and served as control. The thickness of the mouse hind paws was measured with a Vernier Caliper 1, 2, and 3 h after carrageenan challenge. The percentage inhibition of the test compounds as well as diclofenac sodium, used as reference, was calculated relative to carrageenan. The data represent the mean percentage protection ± SEM. *Significant difference from controls at P < 0.05; #significant difference from diclofenac sodium at P < 0 .05.