Figure 2 (a–c) Histopathological results of the testes for the animals in group 2 (250 mg/kg Hibiscus sabdariffa). (a) Loosely packed seminiferous tubules in the testes (H&E, ×40). (b) Germ cell series and arrested development of the spermatozoa (H&E, ×100). (c) Loss of demonstrable spermatogenic series in the testes (H&E, ×400). Sections of (a–c) showed a marked reduction in the seminiferous tubules that are loosely packed, bounded by a thick basement membrane, and showed loss of germ cell series and arrested development at the spermatogonium stage (no demonstrable spermatogenic series). The interstitium showed evidence of replacement fibrosis and prominence of Leydig cells. This indicates the absence of spermatozoa and spermatids and the presence of few spermatogonium.