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  Indian J Med Microbiol
 

Figure 1 In-vitro micropropagation of anise: (a) multiple shoot induction from nodal explants cultured on MS medium supplemented with BAP (5 µmol/l), Kin (1 µmol/l), and NAA (0.5 µmol/l) for 5 weeks; (b) root induction from nodal-derived shoots cultured on MS medium enhanced with IBA (3 µmol/l) for 4 weeks; (c) in-vitro propagated plantlet grown in a pot containing a sterilized mixture of peat moss, garden soil, and sand (1 : 1: 1) for 10 days. BAP, 6-benzylaminopurine; IBA, indole-3-butyric acid; Kin, kinetin; MS, Murashige and Skoog; NAA, naphthalene acetic acid.

Figure 1 <i>In-vitro</i> micropropagation of anise: (a) multiple shoot induction from nodal explants cultured on MS medium supplemented with BAP (5 µmol/l), Kin (1 µmol/l), and NAA (0.5 µmol/l) for 5 weeks; (b) root induction from nodal-derived shoots cultured on MS medium enhanced with IBA (3 µmol/l) for 4 weeks; (c) <i>in-vitro</i> propagated plantlet grown in a pot containing a sterilized mixture of peat moss, garden soil, and sand (1 : 1: 1) for 10 days. BAP, 6-benzylaminopurine; IBA, indole-3-butyric acid; Kin, kinetin; MS, Murashige and Skoog; NAA, naphthalene acetic acid.